+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Sialic acid binding properties of soluble coronavirus spike (S1) proteins: differences between infectious bronchitis virus and transmissible gastroenteritis virus



Sialic acid binding properties of soluble coronavirus spike (S1) proteins: differences between infectious bronchitis virus and transmissible gastroenteritis virus



Viruses 5(8): 1924-1933



The spike proteins of a number of coronaviruses are able to bind to sialic acids present on the cell surface. The importance of this sialic acid binding ability during infection is, however, quite different. We compared the spike protein of transmissible gastroenteritis virus (TGEV) and the spike protein of infectious bronchitis virus (IBV). Whereas sialic acid is the only receptor determinant known so far for IBV, TGEV requires interaction with its receptor aminopeptidase N to initiate infection of cells. Binding tests with soluble spike proteins carrying an IgG Fc-tag revealed pronounced differences between these two viral proteins. Binding of the IBV spike protein to host cells was in all experiments sialic acid dependent, whereas the soluble TGEV spike showed binding to APN but had no detectable sialic acid binding activity. Our results underline the different ways in which binding to sialoglycoconjugates is mediated by coronavirus spike proteins.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 037292639

Download citation: RISBibTeXText

PMID: 23896748

DOI: 10.3390/v5081924


Related references

Coronavirus infectious bronchitis virus partial amino terminal sequencing of spike polypeptide s 2 identifies the sequence arginylarginylphenylalanylarginylarginine at the cleavage site of the spike precursor propolypeptide of infectious bronchitis virus strains beaudette and m 41. Virus Research 4(2): 133-144, 1986

Major receptor-binding and neutralization determinants are located within the same domain of the transmissible gastroenteritis virus (coronavirus) spike protein. Journal of Virology 68(12): 8008-8016, 1994

N-acetylneuraminic acid plays a critical role for the haemagglutinating activity of avian infectious bronchitis virus and porcine transmissible gastroenteritis virus. Advances in Experimental Medicine and Biology 342: 305-310, 1993

Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity. Journal of Virology 70(8): 5634-5637, 1996

Intracellular processing of the porcine coronavirus transmissible gastroenteritis virus spike protein expressed by recombinant vaccinia virus. Virology 182(2): 765-773, 1991

Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins. Veterinary Research 45: 67, 2014

Cultivation techniques for animal coronaviruses: Emphasis on feline infectious peritonitis virus, canine coronavirus, transmissible gastroenteritis virus, and porcine hemagglutinating encephalomyelitis virus. Methods in Cell Science 11(2): 95-100, 1988

Analysis of the sialic acid-binding activity of transmissible gastroenteritis virus. Corona and related viruses: current concepts in molecular biology and pathogenesis 370, 1995

Analysis of the sialic acid-binding activity of the transmissible gastroenteritis virus. Advances in Experimental Medicine and Biology 380: 367-370, 1995

Cellular immune responses of pigs after primary inoculation with porcine respiratory coronavirus or transmissible gastroenteritis virus and challenge with transmissible gastroenteritis virus. Veterinary Immunology & Immunopathology 48(1-2): 35-54, 1995

Comparison of the sialic acid binding activity of transmissible gastroenteritis coronavirus and E. coli K99. Virus Research 75(1): 69-73, 2001

Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins. Veterinary Research 25(1): 29-36, 1994

Antigenic relations between the infectious bronchitis virus of poultry and the transmissible gastroenteritis virus of swine. 1975

Antigenic relationships of avian infectious bronchitis virus and transmissible gastroenteritis virus of pigs. Veterinaria, Mexico 6(2): 38-47, 1975

Is the sialic acid binding activity of the S protein involved in the enteropathogenicity of transmissible gastroenteritis virus?. Advances in Experimental Medicine and Biology 440: 557-561, 1998