+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Comparative genomic analysis of Drosophila melanogaster and vector mosquito developmental genes

Comparative genomic analysis of Drosophila melanogaster and vector mosquito developmental genes

Plos One 6(7): E21504

Genome sequencing projects have presented the opportunity for analysis of developmental genes in three vector mosquito species: Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae. A comparative genomic analysis of developmental genes in Drosophila melanogaster and these three important vectors of human disease was performed in this investigation. While the study was comprehensive, special emphasis centered on genes that 1) are components of developmental signaling pathways, 2) regulate fundamental developmental processes, 3) are critical for the development of tissues of vector importance, 4) function in developmental processes known to have diverged within insects, and 5) encode microRNAs (miRNAs) that regulate developmental transcripts in Drosophila. While most fruit fly developmental genes are conserved in the three vector mosquito species, several genes known to be critical for Drosophila development were not identified in one or more mosquito genomes. In other cases, mosquito lineage-specific gene gains with respect to D. melanogaster were noted. Sequence analyses also revealed that numerous repetitive sequences are a common structural feature of Drosophila and mosquito developmental genes. Finally, analysis of predicted miRNA binding sites in fruit fly and mosquito developmental genes suggests that the repertoire of developmental genes targeted by miRNAs is species-specific. The results of this study provide insight into the evolution of developmental genes and processes in dipterans and other arthropods, serve as a resource for those pursuing analysis of mosquito development, and will promote the design and refinement of functional analysis experiments.

(PDF emailed within 0-6 h: $19.90)

Accession: 037404407

Download citation: RISBibTeXText

PMID: 21754989

DOI: 10.1371/journal.pone.0021504

Related references

Comparative genome analysis of the yellow fever mosquito Aedes aegypti with Drosophila melanogaster and the malaria vector mosquito Anopheles gambiae. Journal of Heredity 95(2): 103-113, 2004

A comparative genomic analysis of two distant diptera, the fruit fly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae. Genome Research 12(1): 57-66, 2002

Characterizing the developmental transcriptome of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) through comparative genomic analysis with Drosophila melanogaster utilizing modENCODE datasets. Bmc Genomics 15: 942, 2015

Evolution of a distinct genomic domain in Drosophila: comparative analysis of the dot chromosome in Drosophila melanogaster and Drosophila virilis. Genetics 185(4): 1519-1534, 2011

Comparative genomic physical mapping of Anopheles gambiae genes with the completed Drosophila melanogaster genome. American Journal of Tropical Medicine & Hygiene 62(3 Suppl.): 219, 2000

Comparative genomic analysis of malaria mosquito vector-associated novel pathogen Elizabethkingia anophelis. Genome Biology and Evolution 6(5): 1158-1165, 2016

Genomic structure and sequence analysis of Drosophila melanogaster HSC70 genes. Gene 128(2): 155-163, 1993

Mapping the pericentric heterochromatin by comparative genomic hybridization analysis and chromosome deletions in Drosophila melanogaster. Genome Research 22(12): 2507-2519, 2013

The generation and analysis of deficiencies within a small genomic region on the X chromosome of Drosophila melanogaster containing two genes, enhancer of rudimentary and CG15352. Fly 1(4): 245-250, 2008

Brain development in the yellow fever mosquito Aedes aegypti: a comparative immunocytochemical analysis using cross-reacting antibodies from Drosophila melanogaster. Development Genes and Evolution 221(5-6): 281-296, 2012

Comparative genomic analysis of novel conserved peptide upstream open reading frames in Drosophila melanogaster and other dipteran species. Bmc Genomics 9: 61, 2008

A comparative analysis of highly conserved sex-determining genes between Apis mellifera and Drosophila melanogaster. Genetics and Molecular Research 5(1): 154-168, 2006

Comparative analysis of the sequence and structure of two Drosophila melanogaster genes encoding vitelline membrane proteins. Gene 136(1-2): 121-127, 1993

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae. Journal of Visualized Experiments 2018(128), 2018

Genomic deletions of the Drosophila melanogaster Hsp70 genes. Genetics 168(3): 1467-1476, 2004