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Full Length cDNA Cloning and Tissue Expression of Major Histocompatibility Complex MHC - II beta in Cobia Rachycentron canadum


, : Full Length cDNA Cloning and Tissue Expression of Major Histocompatibility Complex MHC - II beta in Cobia Rachycentron canadum. Chinese Journal of Zoology 45.4 (Aug 20): 118-128

The homology cloning and RACE PCR were employed to clone the MHC-II beta gene from cobia (Rachycentron canadium). The full length cDNA of MHC-II beta comprises 1 161 bp with a 20 bp 5' untranslated region (UTR), a 394 bp 3' UTR and a 747 bp open reading frame (ORF) encoding a polypeptide of 248 amino acid residues with a predicted molecular weight of 27.99 ku and theoretical isoelectric point of 6.21. The putative protein showed homology varying from 28.5% to 77.5% when compared with other fishes, mouse (Mus musculus), chicken (Gallus gallus) and human (Homo sapiens). The protein sequence showed all the important features including leader peptide, beta 1, beta 2, CP/TM/CYT regions and conserved cysteines. The MHC-II beta expression was detected in all tested tissues with different expression levels. The high expression was detected in head kidney, moderate expression was detected in gill, spleen and intestine, while low expression was detected in heart, brain and muscle.

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Vieira, R.Augusto.Mendonça.; Tedeschi, L.Orlindo.; Cannas, A., 2008: A generalized compartmental model to estimate the fibre mass in the ruminoreticulum: 2. Integrating digestion and passage. Models used to predict digestibility and fill of the dietary insoluble fibre (NDF) treat the ruminoreticular particulate mass as a single pool. The underlying assumption is that escape of particles follows first-order kinetics. In this paper, we p...

Salceda, R.; Pasantes-Morales, H., 1975: Calcium coupled release of [35S]taurine from retina. Brain Research 96(1): 206-211

JACOBSSON, C., 1960: Follow-up examination of dental radiograms of 1957 with examination of corresponding medical journals. Svenska Lakartidningen 57: 2379-2385

Li, T.; Yu, S., 2003: The nature of calcifying odontogenic cysts of the jaws. To clarify the clinicopathological and behavioral spectrum of the so-called calcifying odontogenic cysts (COC). Retrospective analysis of 21 cases previously diagnosed as COC was undertaken to evaluate their clinical, radiographic, pathological an...

Yvon, M., 2006: Key enzymes for flavour formation by lactic acid bacteria. In the special issue: Dairy Science World Series Conference Proceedings on "Cheese Science 2006", held July 24-25, 2006, Sydney, Australia.

Wang, Q.; Fu, A.Z.; Brenner, S.; Kalmus, O.; Banda, H.Thomas.; De Allegri, M., 2015: Out-of-pocket expenditure on chronic non-communicable diseases in sub-Saharan Africa: the case of rural Malawi. In Sub-Saharan Africa (SSA) the disease burden of chronic non-communicable diseases (CNCDs) is rising considerably. Given weaknesses in existing financial arrangements across SSA, expenditure on CNCDs is often borne directly by patients through ou...

Kiel, R.J., 2004: Review: pneumococcal vaccination does not prevent pneumonia or death in adults. Acp Journal Club 141(1): 16-16

Rodskjer, N.; Kornher, A., 1971: On the determination of the radiant energy between the wavelengths 0.3-0.7 micro in plant stands. Equipment for measuring global radiation between 0.3-0.7 jam in crops and methods for using it are described. This radiation parameter is obtained as the difference between the readings of pyranometers, fitted with clear glass and RG8 filters, mov...

MUSOTTO, F.; ROMANO, A., 1951: Effect of streptomycin of antigens of Salmonella typhi. III. Bollettino Della Societa Italiana di Biologia Sperimentale 27(12): 1762-1763

Cloud, Preston E., 1961: Paleobiogeography of the marine realm. Publ Amer Assoc Advance Sci 67: 151-200