+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Full Length cDNA Cloning and Tissue Expression of Major Histocompatibility Complex MHC - II beta in Cobia Rachycentron canadum

, : Full Length cDNA Cloning and Tissue Expression of Major Histocompatibility Complex MHC - II beta in Cobia Rachycentron canadum. Chinese Journal of Zoology 45.4 (Aug 20): 118-128

The homology cloning and RACE PCR were employed to clone the MHC-II beta gene from cobia (Rachycentron canadium). The full length cDNA of MHC-II beta comprises 1 161 bp with a 20 bp 5' untranslated region (UTR), a 394 bp 3' UTR and a 747 bp open reading frame (ORF) encoding a polypeptide of 248 amino acid residues with a predicted molecular weight of 27.99 ku and theoretical isoelectric point of 6.21. The putative protein showed homology varying from 28.5% to 77.5% when compared with other fishes, mouse (Mus musculus), chicken (Gallus gallus) and human (Homo sapiens). The protein sequence showed all the important features including leader peptide, beta 1, beta 2, CP/TM/CYT regions and conserved cysteines. The MHC-II beta expression was detected in all tested tissues with different expression levels. The high expression was detected in head kidney, moderate expression was detected in gill, spleen and intestine, while low expression was detected in heart, brain and muscle.

Accession: 037425027

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

SuJianMing; XiaoTiaoYi; ZhangXueWen; ChenTao; ZhangHuaiYun, 2008: Cloning and tissue expression of full length cDNA containing major histocompatibility complex II alpha from grass carp. The full-length cDNA encoding grass carp MHC class II alpha was cloned from grass carp using cDNA library and RACE PCR approach. The MHC class II alpha was 1007 bp in length, including 34 bp 5' terminal UTR, 717 bp encoding region and 256 bp...

Zhang YuXi; Chen SongLin, 2007: Full length cDNA cloning and tissue expression of major histocompatibility complex (MHC) II a from turbot Scophthalmus maximus. The full-length cDNA encoding turbot MHC class II A was cloned from turbot using homology cloning and RACE PCR method. The MHC class II A was 1131bp in length, including 75bp 5' terminal UTR, 720bp encoding region and 336bp 3' terminal U...

Tsai, M-Ling.; Chen, H-Yung.; Tseng, M-Cheuh.; Chang, R-Chang., 2008: Cloning of peroxisome proliferators activated receptors in the cobia (Rachycentron canadum) and their expression at different life-cycle stages under cage aquaculture. We present the cDNA sequences and tissue mRNA expression of peroxisome proliferator-activated receptor (PPAR) alpha, beta and gamma isotypes in the cobia (Rachycentron canadum), a warm water pelagic fish that is becoming a fish of choice for offsh...

Chuang, W-Hsiao.; Liu, P-Chung.; Hung, C-Yu.; Lee, K-Kau., 2015: Purification, characterization and molecular cloning of alpha-2-macroglobulin in cobia, Rachycentron canadum. Alpha-2-macroglobulin (α-2-M) is a broad spectrum protease inhibitor which is abundant in the plasma of vertebrates and several invertebrates. The α-2-M was purified from cobia (Rachycentron canadum) plasma by a four-step procedure: poly ethylen...

Costa, Gão.W.W.F.; Cioffi,; Bertollo, L.A.C.; Molina, W.F., 2016: Structurally Complex Organization of Repetitive DNAs in the Genome of Cobia (Rachycentron canadum). Repetitive DNAs comprise the largest fraction of the eukaryotic genome. They include microsatellites or simple sequence repeats (SSRs), which play an important role in the chromosome differentiation among fishes. Rachycentron canadum is the only r...

Feng, J.; Su, Y.; Guo, Z.; Xu, L.; Sun, X.; Wang, Y., 2013: Identification and expression analysis of a CC chemokine from cobia (Rachycentron canadum). Chemokines are small, secreted cytokine peptides known principally for their ability to induce migration and activation of leukocyte populations and regulate the immune response mechanisms. The cobia (<i>Rachycentron canadum</i>), a ma...

Yang, Y.; Wang, C.; Zhao, Y.; Yu, X., 2009: Reindeer beta-defensin-1: full-length cDNA cloning and tissue expression. Defensins are members of a large diverse family of cationic antimicrobial peptides that share a signature pattern consisting of six conserved cysteine residues. Here we report the identification of a novel beta-defensin, reindeer beta-defensin-1 (...

Byadgi, O.; Puteri, D.; Lee, Y-Horn.; Lee, J-Wei.; Cheng, T-Chih., 2014: Identification and expression analysis of cobia (Rachycentron canadum) Toll-like receptor 9 gene. Cobia culture is hindered by bacterial infection (Photobacterium damselae subsp. piscicida) and in order to study the effect of P. damselae subsp. piscicida challenge and CpG ODN stimulation on cobia Toll like receptor 9 (RCTLR9), we used PCR to c...

Hurley-Sanders, J.; Harms, C.; Christiansen, E.; Clarke, E.; Law, J., 2015: Exuberant granulation tissue response associated with Neobenedenia sp. (Monogenea: Capsalidae) infestation in two cobia, Rachycentron canadum (Linnaeus). Monogenean parasite infestations are common in captive marine teleosts, and are generally found on the skin and gills. This report describes an unusual pathological presentation of exuberant granulation tissue of the gills, suspected to be related...

Liu, K.; Chi, S.; Liu, H.; Dong, X.; Yang, Q.; Zhang, S.; Tan, B., 2015: Toxic effects of two sources of dietborne cadmium on the juvenile cobia, Rachycentron canadum L. and tissue-specific accumulation of related minerals. In the present study, juvenile cobia, Rachycentron canadum L. were fed diets contaminated by two different sources of cadmium: squid viscera meal (SVM-Cd, organic form) and cadmium chloride (CdCl2-Cd, inorganic form). The Cd concentrations in fish...