EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis



Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis



Parasitology Research 108(1): 211-217



Recent systematic studies of Opisthorchis viverrini based on multilocus enzyme electrophoresis (MEE) have shown that there are at least five genetic groups and possibly two cryptic species occurring in Thailand and Lao PDR each associated with a specific wetland system. A study based on mitochondrial DNA (mtDNA) gene analyses of an O. viverrini population from Savannakhet (SV, Lao PDR) clustered with several isolates from Thailand and Lao PDR although they originated from different river wetland systems. We used MEE to re-examine whether O. viverrini from SV was similar genetically to isolates from Thailand and Lao PDR. The allelic profiles of O. viverrini from SV and five different wetlands representing defined genetic groups of O. viverrini were recorded at 24 enzyme loci as opposed to only two loci of mtDNA. Contrary to previous studies, O. viverrini from SV was found to have fixed genetic differences at six to eight of the 24 loci examined (24.50-35.42%). Allelic data indicated that O. viverrini from SV differed from isolates in the Nam Ngum River wetland in Lao PDR (29.33% fixed genetic differences) and clustered with O. viverrini from Nakhon Phanom and Sakon Nakhon within the Songkram River wetland in Thailand but had fixed genetic differences from these at 24.5% of loci examined. Our data confirm the association between genetic groups of O. viverrini and specific wetland systems, and raise important questions regarding the significance of the genetic differences and relationships of O. viverrini from these wetlands.

(PDF emailed within 0-6 h: $19.90)

Accession: 037426326

Download citation: RISBibTeXText

PMID: 20865431

DOI: 10.1007/s00436-010-2059-8



Related references

Phylogenetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini sensu lato from Sakon Nakhon, Thailand by mitochondrial and nuclear DNA sequencing. Infection, Genetics and Evolution, 2018

Analysis of the population genetics of Opisthorchis viverrini sensu lato in the Nam Ngum River wetland, Lao PDR, by multilocus enzyme electrophoresis. Parasitology Research 113(8): 2973-2981, 2014

Comparison of gene expression profiles between Opisthorchis viverrini and non-Opisthorchis viverrini associated human intrahepatic cholangiocarcinoma. Hepatology 44(4): 1025-1038, 2006

Function association of enhanced expressed genes between Opisthorchis viverrini and non Opisthorchis viverrini-related cholangiocarcinoma. Revista Espanola de Enfermedades Digestivas 100(7): 439-440, 2008

Patterns of trematode infections of Opisthorchis viverrini (Opisthorchiidae) and Haplorchis taichui (Heterophyidae) in human populations from two villages in Savannakhet Province, Lao PDR. Journal of Helminthology 89(4): 439-445, 2016

Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis. Parasitology 96: 119-128, 1988

Viable metacercariae of Opisthorchis viverrini in northeastern Thai cyprinid fish dishes--as part of a rational program for control of O. viverrini-associated cholangiocarcinoma. Parasitology Research 112(3): 1323-1327, 2013

Genetic variation at three enzyme loci within a Thailand population of Opisthorchis viverrini. Parasitology Research 103(6): 1283-1287, 2008

Opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like. Acta Tropica 140: 34-40, 2015

Evidence of a species complex within the food-borne trematode Opisthorchis viverrini and possible co-evolution with their first intermediate hosts. International Journal for Parasitology 37(6): 695-703, 2007

Specific monoclonal antibodies to Opisthorchis viverrini. International Journal for Parasitology 21(8): 969-974, 1991

Specific gravity of Opisthorchis viverrini eggs. Journal of Helminthology 72(4): 359-361, 1998

Serodiagnosis of opisthorchis viverrini infestation by an enzyme immuno-assay. Tropenmedizin und Parasitologie 33(1): 8-10, 1982

Enzyme markers to identify and characterize Opisthorchis viverrini in Thailand and Lao PDR. Southeast Asian Journal of Tropical Medicine and Public Health 37 Suppl 3: 43-47, 2007

Species-specific primers designed from RAPD products for Bithynia funiculata, the first intermediate host of liver fluke, Opisthorchis viverrini, in North Thailand. Journal of Parasitology 99(3): 433-437, 2013