EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

CbfA, the C-module DNA-binding factor, plays an essential role in the initiation of Dictyostelium discoideum development






Eukaryotic Cell 3(5): 1349-1358

CbfA, the C-module DNA-binding factor, plays an essential role in the initiation of Dictyostelium discoideum development

We recently isolated from Dictyostelium discoideum cells a DNA-binding protein, CbfA, that interacts in vitro with a regulatory element in retrotransposon TRE5-A. We have generated a mutant strain that expresses CbfA at <5% of the wild-type level to characterize the consequences for D. discoideum cell physiology. We found that the multicellular development program leading to fruiting body formation is highly compromised in the mutant. The cells cannot aggregate and stay as a monolayer almost indefinitely. The cells respond properly to prestarvation conditions by expressing discoidin in a cell density-dependent manner. A genomewide microarray-assisted expression analysis combined with Northern blot analyses revealed a failure of CbfA-depleted cells to induce the gene encoding aggregation-specific adenylyl cyclase ACA and other genes required for cyclic AMP (cAMP) signal relay, which is necessary for aggregation and subsequent multicellular development. However, the cbfA mutant aggregated efficiently when mixed with as few as 5% wild-type cells. Moreover, pulsing cbfA mutant cells developing in suspension with nanomolar levels of cAMP resulted in induction of acaA and other early developmental genes. Although the response was less efficient and slower than in wild-type cells, it showed that cells depleted of CbfA are able to initiate development if given exogenous cAMP signals. Ectopic expression of the gene encoding the catalytic subunit of protein kinase A restored multicellular development of the mutant. We conclude that sensing of cell density and starvation are independent of CbfA, whereas CbfA is essential for the pattern of gene expression which establishes the genetic network leading to aggregation and multicellular development of D. discoideum.


Accession: 038054916

PMID: 15470262

DOI: 10.1128/EC.3.5.1349-1358.2004



Related references

Bilzer, A.; Dölz, H.; Reinhardt, A.; Schmith, A.; Siol, O.; Winckler, T., 2011: The C-module-binding factor supports amplification of TRE5-A retrotransposons in the Dictyostelium discoideum genome. Retrotransposable elements are molecular parasites that have invaded the genomes of virtually all organisms. Although retrotransposons encode essential proteins to mediate their amplification, they also require assistance by host cell-encoded mach...

Morio, T.; Yasukawa, H.; Urushihara, H.; Saito, T.; Ochiai, H.; Takeuchi, I.; Maeda, M.; Tanaka, Y., 2001: FebA: A gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum. We have identified a gene encoding a eukaryotic initiation factor 4E-binding protein (4E-BP) in the EST database of the Dictyostelium cDNA project. The Dictyostelium 4E-BP, designated febA (four e-binding), showed significant similarity to mammali...

Morio, T.; Yasukawa, H.; Urushihara, H.; Saito, T.; Ochiai, H.; Takeuchi, I.; Maeda, M.; Tanaka, Y., 2001: FebA: a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum. We have identified a gene encoding a eukaryotic initiation factor 4E-binding protein (4E-BP) in the EST database of the Dictyostelium cDNA project. The Dictyostelium 4E-BP, designated febA (four e-binding), showed significant similarity to mammali...

IIjima, N.; Takagi, T.; Maeda, Y., 1994: Purification and identification of a secreted factor required for the initiation of development in Dictyostelium discoideum. Zoological Science (Tokyo) 11(SUPPL ): 62

Smith, T.S.; Pineda, J.M.; Donaghy, A.C.; Damer, C.K., 2010: Copine A plays a role in the differentiation of stalk cells and the initiation of culmination in Dictyostelium development. Copines are calcium-dependent phospholipid-binding proteins found in diverse eukaryotic organisms. We are studying the function of copines in Dictyostelium discoideum, a single-celled amoeba that undergoes cell differentiation and morphogenesis to...

Gosain, A.; Srivastava, A.; Saran, S., 2014: Peptide: N- glycanase is expressed in prestalk cells and plays a role in the differentiation of prespore cells during development of Dictyostelium discoideum. Peptide: N-glycanase (PNGase) enzyme is found throughout eukaryotes and plays an important role in the misfolded glycoprotein degradation pathway. This communication reports the expression patterns of the pngase transcript (as studied by the analy...

Pang, T-Ling.; Wu, C-Jung.; Chen, P-An.; Weng, Y-Lan.; Chen, M-Yu., 2007: Dictyostelium gnt15 encodes a protein with similarity to LARGE and plays an essential role in development. LARGE is a putative glycosyltransferase found to be mutated in mice with myodystrophy or patients with congenital muscular dystrophy. By homology searches, we identified in the Dictyostelium discoideum genome four open reading frames, i.e. gnt12-...

Menon, B.; Gulappa, T.; Menon, K.M.J., 2015: Eukaryotic initiation factor 5A plays an essential role in luteinizing hormone receptor regulation. Down-regulation of LH receptor (LHR) in the ovary by its ligand is mediated by a specific RNA-binding protein, designated LH receptor mRNA-binding protein (LRBP), through translational suppression and mRNA degradation. Using yeast 2-hybrid screens...

Tokusumi, Y.; Zhou, S.; Takada, S., 2004: Nuclear respiratory factor 1 plays an essential role in transcriptional initiation from the hepatitis B virus x gene promoter. The X gene of hepatitis B virus (HBV) is one of the major factors in HBV-induced hepatocarcinogenesis and is essential for the establishment of productive HBV replication in vivo. Recent studies have shown that the X gene product targets mitochond...

Wessels, D.; Brincks, R.; Kuhl, S.; Stepanovic, V.; Daniels, K.J.; Weeks, G.; Lim, C.J.; Spiegelman, G.; Fuller, D.; Iranfar, N.; Loomis, W.F.; Soll, D.R., 2004: RasC plays a role in transduction of temporal gradient information in the cyclic-AMP wave of Dictyostelium discoideum. To define the role that RasC plays in motility and chemotaxis, the behavior of a rasC null mutant, rasC-, in buffer and in response to the individual spatial, temporal, and concentration components of a natural cyclic AMP (cAMP) wave was analyzed...