EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

CbfA, the C-module DNA-binding factor, plays an essential role in the initiation of Dictyostelium discoideum development


, : CbfA, the C-module DNA-binding factor, plays an essential role in the initiation of Dictyostelium discoideum development. Eukaryotic Cell 3(5): 1349-1358

We recently isolated from Dictyostelium discoideum cells a DNA-binding protein, CbfA, that interacts in vitro with a regulatory element in retrotransposon TRE5-A. We have generated a mutant strain that expresses CbfA at <5% of the wild-type level to characterize the consequences for D. discoideum cell physiology. We found that the multicellular development program leading to fruiting body formation is highly compromised in the mutant. The cells cannot aggregate and stay as a monolayer almost indefinitely. The cells respond properly to prestarvation conditions by expressing discoidin in a cell density-dependent manner. A genomewide microarray-assisted expression analysis combined with Northern blot analyses revealed a failure of CbfA-depleted cells to induce the gene encoding aggregation-specific adenylyl cyclase ACA and other genes required for cyclic AMP (cAMP) signal relay, which is necessary for aggregation and subsequent multicellular development. However, the cbfA mutant aggregated efficiently when mixed with as few as 5% wild-type cells. Moreover, pulsing cbfA mutant cells developing in suspension with nanomolar levels of cAMP resulted in induction of acaA and other early developmental genes. Although the response was less efficient and slower than in wild-type cells, it showed that cells depleted of CbfA are able to initiate development if given exogenous cAMP signals. Ectopic expression of the gene encoding the catalytic subunit of protein kinase A restored multicellular development of the mutant. We conclude that sensing of cell density and starvation are independent of CbfA, whereas CbfA is essential for the pattern of gene expression which establishes the genetic network leading to aggregation and multicellular development of D. discoideum.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 038054916

PMID: 15470262

DOI: 10.1128/EC.3.5.1349-1358.2004

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Jargin, S.V., 2007: Over-estimation of radiation-induced malignancy after the Chernobyl accident. It was the aim of the current study to evaluate the utility of human placental alkaline phosphatase (hPLAP) as a genetic marker for cell tracking in bone and cartilage, using transgenic Fischer 344 rats expressing hPLAP under the control of the ub...

Gray, Norman M.; Cameron, Janet M.; Cammack, N.; Cobley, Kevin N.; Holmes, Duncan S.; Humber, David C.; Orr, David C.; Penn, Charles R.; Potter, Rachel, 1994: Discovery and analysis of a series of C-2-symmetric HIV-1 proteinase inhibitors derived from penicillin. In order to identify a suitable peptide substrate for human immunodeficiency virus-1 (HIV-1) proteinase, a range of peptides from various cleavage sites within the gag-pol polyprotein were assayed by HPLC for specific cleavage. The peptide with th...

Ahlberg Per E., 1996: Morphological experimentation during the origin of tetrapods. Journal of Vertebrate Paleontology 16(3, Suppl

Berger E.M.; Murphy K.; Shibao G.A.; Repine J.E., 1988: Small increases in ph decrease uptake of escherichia coli by human neutrophils in vitro. Increases in pH from 7.4 to 7.8 decreased the ability of human neutrophils in serum to ingest and kill Escherichia coli ATCC 29552 in vitro. In contrast, similar increases in pH did not decrease the bactericidal activity of neutrophils in serum ag...

Miller Marti L.; Bradley Dwight C.; Bundtzen Thomas K.; Blodgett Robert B.; Pessagno Emile A.; Tucker Robert; Harris Anita G., 2005: Age and provenance of the restricted Gemuk Group Triassic to Lower Cretaceous, Southwest Alaska. The restricted Gemuk Group is an Upper Triassic to Lower Cretaceous deep marine succession in southwest Alaska. First defined by Cady et al. in 1955, the Gemuk Group quickly became a regional catch-all unit that ended up as part of four different...

Pereda Suberbiola, Javier; Galton, Peter M., 1990: On the taxonomic status of the dinosaur Struthiosaurus austriacus Bunzel from the Late Cretaceous of Austria. The fragmentary skull of Struthiosaurus austriacus Bunzel from the Campanian Gosau Beds of Austria, recently regarded as a theropod dinosaur, is reinterpreted as that of an immature nodosaurid ankylosaur. Struthiosaurus is definitively characteriz...

Morgan, K.; Conklin, D.; Pawson, A.J.; Sellar, R.; Ott, T.R.; Millar, R.P., 2003: A transcriptionally active human type II gonadotropin-releasing hormone receptor gene homolog overlaps two genes in the antisense orientation on chromosome 1q.12. GnRH-II peptide hormone exhibits complete sequence conservation across vertebrate species, including man. Type-II GnRH receptor genes have been characterized recently in nonhuman primates, but the human receptor gene homolog contains a frameshift,...

Carew, Lb; Chamberlain, Vm, 2001: Interdisciplinary update nutrition course offered to educators through interactive television. Journal of nutrition education and behavior 33(6): 352-353

Cheriyath, Venugopalan; Novina, Carl D.; Roy, Ananda L., 1998: TFII-I regulates Vb promoter activity through an initiator element. Molecular and Cellular Biology 18(8): 44-54

Domashuk Yu, 1988: Tolerance of positive gz acceleration of pilots with health abnormalities. Fighter pilots with health abnormalities were examined for their tolerance to +Gz acceleration. It was found that pilots with psychoneurological disorders and cardiac arrhythmias showed a lower acceleration tolerance. Pilots with arterial hyperten...