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Comparison of DNA extraction protocols in Lernaea sp Copepoda Cyclopoida Comparacao de protocolos para extracao do DNA de Lernaea sp Copepoda Cyclopoida


Acta Scientiarum Animal Sciences. Jul-Dec; 252: 219-222
Comparison of DNA extraction protocols in Lernaea sp Copepoda Cyclopoida Comparacao de protocolos para extracao do DNA de Lernaea sp Copepoda Cyclopoida
In this study thirty samples of adult females of Lernaea sp. were collected from naturally infested fishes at two fisheries in northern Parana, Brazil. Two protocols of DNA extraction were tested for parasites tissues. The results have shown that the protocol using Kit Puregene DNA Isolation (GENTRA) was adequate for genomic DNA extraction in Lernaea. The methodology using CTAB protocol, described by Black IV et al. (1996), modified for DNA extraction in Lernaea sp., did not work properly.

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Accession: 038088104



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