EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Comparison of different diagnostic methods for detection of Isospora suis in faeces of suckling piglets Vergleich verschiedener Verfahren zur Untersuchung von Saugferkelkot auf Isospora suis






Berliner und Muenchener Tieraerztliche Wochenschrift. July-August; 1197-8: 282-286

Comparison of different diagnostic methods for detection of Isospora suis in faeces of suckling piglets Vergleich verschiedener Verfahren zur Untersuchung von Saugferkelkot auf Isospora suis

A highly sensitive diagnostic method is of great importance for detection of I.suis (Isospora suis). In the present study KSFV (combined sedimentation-flotation method) using different flotation solutions (NaCl, ZnSO4, NaCl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to FV (flotation method) using NaCl-sucrose solution and to AM (autofluorescence microscopy). Ninety faecal samples were examined. Using AM, oocysts were detected in 83.3% of the samples. In cases of KSFV and FV, respectively, 41.1% to 53.3% of samples were tested positive. The quantity of detected oocysts was markedly higher in AM than in KSFV or FV. Therefore AM is the most sensitive of the comprised methods. The expenditure of labour is lowest in AM,time exposure is lower in FV than in KSFV. For performance of AM a fluorescence microscope is required contrary to KSFV and FV.


Accession: 038088642



Related references

Kuhnert, Y.; Schmaeschke, R.; Daugschies, A., 2006: Vergleich verschiedener Verfahren zur Untersuchung von Saugferkelkot auf Isospora suis. A highly sensitive diagnostic method is of great importance for detection of I.suis (Isospora suis). In the present study KSFV (combined sedimentation-flotation method) using different flotation solutions (NaCl, ZnSO4, NaCl-sucrose) was investigat...

Kuhnert, Y.; Schmaschke, R.; Daugschies, A., 2006: Comparison of different diagnostic methods for detection of Isospora suis in faeces of suckling piglets. A highly sensitive diagnostic method is of great importance for the detection of Isospora suis. In the present study, KSFV (combined sedimentation-flotation method) using different flotation solutions (NaCl, ZnSO4 and NaCl-sucrose) was investigate...

Kuhnert, Y.; Schmäschke, R.; Daugschies, A., 2006: Comparison of different methods for examining the feces of suckling piglets for Isospora suis. A highly sensitive diagnostic method is of great importance for detection of I. suis (Isospora suis). In the present study KSFV (combined sedimentation-flotation method) using different flotation solutions (NaCl, ZnSO4, NaCl-sucrose) was investiga...

Anonymous, 2007: Isospora suis- and Clostridium perfringens as mixed infection in suckling piglets just after post parturn? FT Isospora suis- und Clostridium perfringens- Mischinfektion beim Saugferkel unmittelbar post partum?

Anonymous, 2007: Isospora suis invasion in piglets FT Inwazja Isospora suis it prosiat

Karamon, J.; Ziomko, I.; Cencek, T.; Sroka, J., 2008: Modified flotation method with the use of Percoll for the detection of Isospora suis oocysts in suckling piglet faeces. The modification of flotation method for the examination of diarrhoeic piglet faeces for the detection of <i>Isospora suis</i> oocysts was elaborated. The method was based on removing fractions of fat from the sample of faeces by centr...

Joachim, A.; Ruttkowski, B.; Zimmermann, M.; Daugschies, A.; Mundt, H-C., 2004: Detection of Isospora suis (Biester and Murray 1934) in piglet faeces--comparison of microscopy and PCR. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health 51(3): 140-142

Joachim, A.; Ruttkowski, B.; Zimmermann, M.; Daugschies, A.; Mundt, H.C., 2004: Detection of Isospora suis (Biester and Murray 1934) in piglet faeces - comparison of microscopy and PCR. Forty-four faecal samples from piglets (2-3 animals per group) experimentally infected with Isospora suis were examined 4, 5, 7, 10, and 11 days after infection either with a modified flotation technique followed by McMaster counting (MM), MM comb...

Gualdi, V.; Vezzoli, F.; Luini, M.; Nisoli, L., 2003: The role of Isospora suis in the aetiology of diarrhoea in suckling piglets. A study was conducted to evaluate the role of Isospora suis in the aetiology of diarrhoea in suckling piglets. Litters with characteristic symptoms of coccidiosis from different delivery rooms were selected for the study. Litters with no enteric s...

Gualdi, V.; Vezzoli, F.; Luini, M.; Nisoli, L., 2003: The role of Isospora suis in the ethiology of diarrhoea in suckling piglets. Various studies have confirmed the notable spread of Isospora suis in Italy but the role of the primary pathogen in enteric diseases during the neonatal period remained unclear . In this study, wide ranging investigations (parasitology, bacteriolo...