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Comparison of four microscopic techniques for the diagnosis of leptospirosis in wild rodents in the rural area of Valdivia, Chile Comparacion de cuatro tecnicas microscopicas para el diagnostico de leptospirosis en roedores silvestres en el area rural de Valdivia, Chile



Comparison of four microscopic techniques for the diagnosis of leptospirosis in wild rodents in the rural area of Valdivia, Chile Comparacion de cuatro tecnicas microscopicas para el diagnostico de leptospirosis en roedores silvestres en el area rural de Valdivia, Chile



Revista Latinoamericana de Microbiologia. Julio-Septiembre; 373: 267-272



Kidneys of six different species of 93 wild rodents captured in the rural area of Valdivia (Chile) were simultaneously examined for leptospira infection by means of Levaditi silver stain, dark ground microscopy in wet smears, and immunofluorescence and immunoperoxidase techniques using pooled antiserum against hardjo and pomona serovars. Leptospira was shown to be present in 40 (43.0%) rodents. They were detected in five of the six species: Akodon olivaceus, Akodon longipilis, Rattus rattus, Oryzomis longicaudatus and Mus musculus. Levaditi's technique detected the highest number of positive samples (67.5%) and the dark field microscopy the lowest (32.5%). The detection of leptospira in the kidneys of these wild rodents suggests that their urine may play an important role in the dissemination of leptospirosis in this area of Valdivia.

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