Enzymatic resistance detection by synergist products in a field Tetranychus urticae Koch strain Acari Tetranychidae Deteccion de resistencia enzimatica por productos sinergistas en una linea de campo de Tetranychus urticae Koch Acari Tetranychidae
Cerna, E.; Landeros, J.; Guerrero, E.; Flores, A.E.; Badii, MH.
Folia Entomologica Mexicana. Diciembre; 443: 287-295
Collections of two spotted mites were made in strawberry production fields in the state of Guanajuato, Mexico. The collected material was taken to the laboratory of acarology in the University Autonoma Agraria Antonio Narro. Several bioassay were conducted to determine the resistance proportion, cotoxicity coefficient and to determine the enzymatic resistance mechanisms, using a T urticae susceptible line how reference (Narro susceptible, L-NS). For made the bioassays using the leaf immersion technique. The results indicated that the resistance proportion among field strain and susceptible line was 57.4, 8.0, 11.2, 11.3 y 9.0 times plus the miticides avermectin, bitentrin, dicofol, feributatin oxide and naled respectively. For other side the mixture of synergist piperonyl butoxide (PB)plus the miticides avermectin, bifentrin, dicofol, fenbutatin oxide and naled increased their toxicity in 7.86, 3.69, 10.78, 2.45 and 4.79 times respectively. Using the synergist SSS-tributylphosphorotrithioate (DEF) the increase was 2.15, 6.65, 5.54, 1.73 and 2.65 times; and diethyl maleate (DEM) had 3.17, 2.44, 3.90, 1.95 and 6.34 times more tocixity for the same miticides. The highest values of synergism were obtained with the pyperonil butoxide, which indicate that the greater cause of physiological resistance for T urticae is due to the oxidase enzymes.