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Freshwater prawn Macrobrachium rosenbergii genetic material cryopreservation Criopreservacao de Material Genetico do Camarao-de-Agua-Doce Macrobrachium rosenbergii



Freshwater prawn Macrobrachium rosenbergii genetic material cryopreservation Criopreservacao de Material Genetico do Camarao-de-Agua-Doce Macrobrachium rosenbergii



Revista Brasileira de Zootecnia. November-December; 296: 2157-2161 (Supplement 2



The aim of this work was to evaluate cryopreservation of the genetic material of the freshwater prawn Macrobrachium rosenbergii through two techniques: spermatophore storage (attained through eletroejaculation) in liquid nitrogen, diluted in egg yolk solution added with sodium citrate and dimethyl sulfoxide (DHSO) during 60 days and stocking newly pond-reared prawns in freezer (-13deg. C) during periods of one, three, five, seven and twelve weeks, using the animal body to prevent sperm cells against freezing effects. Newly extruded spermatophores were used as control. The percentage average of viable spermatozoa obtained through freezing in liquid nitrogen, freezer and the unfrozen control group were 64.8, 79.2 and 90.8%, respectively, that showed difference among themselves. The animal's storage in freezer technique showed no correlation between the tested periods and the resulting spermatic viabilities. Sperm viability attained by animal storage in freezer was higher than the one through stocking spermatophores in liquid nitrogen. Both techniques provided the maintenance of viable spermatozoa stock during at least twelve weeks viewing artificial insemination handlings.

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Accession: 038265045

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