Sampling and evaluation of techniques for isolation of native entomopathogenic nematodes obtained in Lavras, Minas Gerais Amostragem e avaliacao de tecnicas para isolamento de nematoides entomopatogenicos nativos obtidos em Lavras, Minas Gerais
Molina Acevedo, J.P.; Moino Jr, A.; Cavalcanti, R.S.; Dolinski, C.; Carvalho, F.A.
Nematologia Brasileira. Junho; 291: 17-23
For some years, entomopathogenic nematodes (EPNs) have been used as biological agents for insect pest control, being a promising tool for Integrated Pest Management programs in Brazil. These nematodes are abundant in many areas around the world, from arctic to tropical zones, being adapted to many insects species due to co-evolution. The exploration of new areas, in the case of tropical areas such as Brazil, and the adoption of effective techniques for the extraction of these biocontrol organisms, need to be adjusted. Therefore, the objectives of this work were to sample and isolate native entomopathogenic nematodes in the area of Lavras, MG and to evaluate the efficiency of isolation techniques such as Baermann funnel and insect trap. In the sampling of native nematodes, three isolates were recovered and named JPM3, JPM3.1 and JPM4. These isolates corresponded to 6.8% of the total samples in areas with pumpkin and weeds. The best isolation technique was insect trap, with 50% efficiency. This study demonstrates the high incidence of EPNs in a small area and that insect trap is the best isolation method.