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Structure of the monosialyl oligosaccharides derived from sa-livary gland mucin glycoproteins of the Chinese swiftlet genus Collocalia Characterization of novel types of extended core structure, Gal1-3 1-6 GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc



Structure of the monosialyl oligosaccharides derived from sa-livary gland mucin glycoproteins of the Chinese swiftlet genus Collocalia Characterization of novel types of extended core structure, Gal1-3 1-6 GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc GalNAc



Journal of Biological Chemistry 26214: 6650-6657



The mucus glycoproteins, the so-called nest-cementing substance, from the salivary gland of Chinese swiflets (genus Collocalia) are mainly constituted of sialic acid-rich O-glycosylproteins. Alkaline reductive treatment of the crude material led to the release of some neutral and numerous monosialyl and disialyl oligosaccharides. These were fractionated by gel filtration, anion-exchange chromatography, and high-performance liquid chromatography. The structures of the monosialyl oligosaccharides were established by combination of sugar and methylation analysis, fast atom bombardment-mass spectrometry, and electron impact-mass spectrometry after permethylation and 1H NMR spectroscopy (at 500 MHz). Typically, some of the monosialyl oligosaccharides appeared to possess the core structure Gal.beta. (1.fwdarw.3) GlcNAc.beta.(1.fwdarw.6) GalNAc.alpha.(1.fwdarw.3)GalNAc-ol. Moreover, the (1.fwdarw.6)-linked branch consisted of an unusual di- or trigalactosyl sequence, Gal.alpha.(1.fwdarw.4)0-1Gal.beta.(1.fwdarw.4)Gal.beta.(1.fwdarw.4)GlcNAc.beta.(1.fwdarw.6) .

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