EurekaMag
+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

ATP-dependent activation of L cell glucocorticoid receptors to the steroid binding form


ATP-dependent activation of L cell glucocorticoid receptors to the steroid binding form



Journal of Biological Chemistry 254(11): 4772-4778



ISSN/ISBN: 0021-9258

PMID: 108283

The specific glucocorticoid binding capacity in cytosols prepared from L929 mouse fibroblasts (L cells) is inactivated with a half-life of approximately 2 h at 25 degrees C. As previously published, this inactivation can be prevented with 10 mM molybdate and markedly slowed by addition of other phosphatase inhibitors such as glucose 1-phosphate and fluoride. We have now found that ATP (5 to 10 mM) also slows the rate of this inactivation. After extensively inactivating the receptor by preincubating cytosol at 25 degrees C for 4 and preventing further inactivation by addition of molybdate, addition of ATP results in reactivation of the steroid binding capacity. Maximal reactivation of 40 to 70% is achieved with 5 to 10 mM ATP. The activation is temperature-dependent and specific for ATP. ADP, GTP, CTP, and UTP do not cause activation and preliminary results indicate no effect of cyclic nucleotides in this system. If activation is prevented by addition of 10 mM EDTA to the cytosol, addition of 3 to 10 mM magnesium permits ATP-dependent activation of the binding capacity. The level of reactivation can be enhanced by addition of a heat-stable factor prepared from the same L cell supernatant. These results support the proposal that L cell glucocorticoid receptors can be activated to the glucocorticoid binding state by an ATP-dependent phosphorylation mechanism.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 038907764

Download citation: RISBibTeXText

Related references

Activation of thymocyte glucocorticoid receptors to the steroid binding form. The roles of reduction agents, ATP, and heat-stable factors. Journal of Biological Chemistry 254(11): 4779-4789, 1979

Dissociation of steroid binding to receptors and steroid induction of biological activity in a glucocorticoid-responsive cell. Endocrinology 112(2): 601-609, 1983

Nadph dependent steroid binding by mammary cytoplasmic glucocorticoid receptors. Biochemical Archives 3(1): 103-112, 1987

Steroid binding activity is retained in a 16-kDa fragment of the steroid binding domain of rat glucocorticoid receptors. Journal of Biological Chemistry 264(24): 14493-14497, 1989

Steroid binding to hepatoma tissue culture cell glucocorticoid receptors involves at least two sulfhydryl groups. Journal of Biological Chemistry 263(29): 15217-15225, 1988

Steroid binding to hepatoma tissue culture cell glucocorticoid receptors at involves at least two sulhydryl groups. The Journal of Biological Chemistry 263(29): 15217-15225, 1988

The Dual Dose-Dependent Effects of Corticosterone on Hippocampal Cell Apoptosis After Traumatic Brain Injury Depend on the Activation Ratio of Mineralocorticoid Receptors to Glucocorticoid Receptors. Frontiers in Pharmacology 12: 713715, 2021

Activation of the dioxin and glucocorticoid receptors to a DNA binding state under cell-free conditions. Journal of Biological Chemistry 265(4): 2269-2277, 1990

Early events in glucocorticoid action. Activation of the steroid receptor and its subsequent specific nuclear binding studied in a cell-free system. Journal of Biological Chemistry 248(16): 5866-5872, 1973

Glucocorticoid receptors and the cell cycle: evidence that the accumulation of glucocorticoid receptors during the S phase of the cell cycle is dependent on ribonucleic acid and protein synthesis. Endocrinology 110(5): 1653-1662, 1982

Mechanism of activation of steroid receptors: involvement of low molecular weight inhibitor in activation of androgen, glucocorticoid, and estrogen receptor systems. Endocrinology 106(4): 1142-1148, 1980

Competitive binding studies with glucocorticoid receptors from rat-thymus cells: differential temperature-dependence of steroid binding. Molecular and Cellular Endocrinology 13(1): 83-92, 1979

Towards a molecular understanding of steroid binding to glucocorticoid receptors. Roy, A K And J H Clark (Ed ) Gene Regulation By Steroid Hormones; Fourth Meadow Brook Conference on Hormones, Fall 1988 Xi+239p Springer-Verlag New York, Inc : Secaucus, New Jersey, Usa; Berlin, West Germany Illus 1-22, 1989

Role of cysteines 640, 656, and 661 in steroid binding to rat glucocorticoid receptors. Journal of Biological Chemistry 267(16): 11366-11373, 1992

Identification of hormone-interacting amino acid residues within the steroid-binding domain of the glucocorticoid receptor in relation to other steroid hormone receptors. Journal of Biological Chemistry 263(14): 6842-6846, 1988