EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Fluorochrome staining of multilamellar liposomes



Fluorochrome staining of multilamellar liposomes



Stain Technology 53(6): 307-310



Multilamellar liposomes can be stained with such fluorochromes as acridine orange, eosin Y, neutral red, and thiazine red. The liposomes are brought into a 1% solution of the fluorochrome; 5-10 minutes later they are centrifuged and washed by resuspending in water or phosphate buffered saline three times. The last pellet is resuspended and a drop studied with the fluorescence microscope (1000 x magnification). The fluorochrome is seen to be accumulated in the liposomal membranes. Acridine orange could also be trapped in the aqueous compartments of the liposomes but the trapped fluorochrome was gradually lost from the liposomes. Part of the fluorochrome, however, remained associated with the liposomal membranes for a long time. Additional experiments justify the conclusion that an equilibrium is maintained between fluorochromes in the aqueous and lipid phases.

(PDF same-day service: $19.90)

Accession: 038940610

Download citation: RISBibTeXText

PMID: 89716

DOI: 10.3109/10520297809111951



Related references

Vital fluorochrome staining of animal cell nuclei with a new fluorochrome. Die Naturwissenschaften 56(5): 286-286, 1969

Liposomes in immunology: multilamellar phosphatidylcholine liposomes as a simple, biodegradable and harmless adjuvant without any immunogenic activity of its own. Immunological Communications 9(3): 243-256, 1980

A karyotype comparison of nine species of aneuploid Zamia by using the conventional orcein staining and the fluorochrome CMA-DAPI differential staining methods. Cytologia 64(4): 449-458, 1999

Formulation of HP-228 in multilamellar liposomes. Pharmaceutical Research (New York) 12(9 SUPPL ): S261, 1995

Plasmid entrapping multilamellar liposomes. Official Gazette of the United States Patent & Trademark Office Patents 1209(4): 3191, April 28, 1998

Effects of polyene antibiotics on multilamellar liposomes i. Korean Biochemical Journal 23(1): 90-96, 1990

Resistive-pulse detection of multilamellar liposomes. Langmuir 28(19): 7572-7577, 2012

Development and characterization of multilamellar liposomes containing pyridostigmine. Pharmaceutical Development and Technology 19(4): 454-459, 2014

Interaction of sea cucumber saponins with multilamellar liposomes. Chemico-Biological Interactions 52(2): 185-202, 1984

Structural characteristics of phospholipid multilamellar liposomes. Journal of Pharmaceutical Sciences 73(1): 122-125, 1984

Multilamellar structures of DNA complexes with cationic liposomes. Biophysical Journal 73(4): 1842-1846, 1997

Effects of polyene antibiotics on multilamellar liposomes ii. Korean Biochemical Journal 23(1): 97-102, 1990

The cryoprotection of multilamellar liposomes by carboxylate anions. Journal of Pharmacy & Pharmacology 40(SUPPL): 27P, 1988

Cholesterol inhibits peroxidation of egg phosphatidylcholine in multilamellar liposomes. Ceska A Slovenska Farmacie 51(5): 240-243, 2002

Characterization of a third phase transition in multilamellar dipalmitoyllecithin liposomes. Biochemistry 20(20): 5707-5710, 1981