Home
  >  
Section 39
  >  
Chapter 38,944

Hemolytic assay of complement and its components from Syrian hamster (Mesocricetus auratus) and Mongolian gerbil (Mariones unguiculatus)

Barta, O.; Klei, T.R.

Developmental and Comparative Immunology 2(3): 519-528

1978

ISSN/ISBN: 0145-305X
PMID: 28965
DOI: 10.1016/s0145-305x(78)80012-3
Accession: 038943966
Download citation:  
Text
  |  
BibTeX
  |  
RIS
Syrian hamster sera may be assayed for hemolytic complement (C) activity by standard procedures with sensitized sheep erythrocytes as used for human sera, but mongolian gerbil sera had higher complement titers when tested with sensitized guinea pig erythrocytes as target cells. The optimal conditions for in vitro testing of hamster C were pH 7.3, ionic strength 0.15, and presence of 1 micrometer of Mg2+ and 0.3 micrometer Ca2+. For gerbil C the optimum pH was 8, ionic strength 0.074, and the same concentration of Mg2+ and Ca2+ as for hamster. Incubation at 37 degrees C for 60 minutes yielded optimal results. Complement components in the serum of both species could be tested with commercially prepared intermediates of sheep erythrocytes pretreated with guinea pig C1 and human C4 respectively, using purified human C2 through 9 and standard methods applied for testing human C components. Both species have all nine components of the complement system.

PDF emailed within 0-6 h: $19.90




Related References

Irmer, S.; Wissdorf, H. 1980: Facial bones of the mongolian gerbil meriones unguiculatus syrian hamster mesocricetus auratus and mouse mus musculus Zeitschrift fuer Versuchstierkunde 22(5-6): 282-295
Wissdorf, H.; Irmer, S. 1979: The skeleton of the hind limb of the mongolian gerbil meriones unguiculatus the syrian hamster mesocricetus auratus and the laboratory mouse mus musculus Zeitschrift fuer Versuchstierkunde 21(3): 163-176
Wissdorf, H.; Irmer, S. 1979: Osteological findings on the thorax of the mongolian gerbil meriones unguiculatus in comparison with that of the syrian hamster mesocricetus auratus and the laboratory mouse mus musculus Zeitschrift fuer Versuchstierkunde 21(1): 17-20
Irmer, S.; Wissdorf, H. 1979: Osteologic findings on the skeleton of the shoulder of the mongolian gerbil meriones unguiculatus in comparison with that of the syrian hamster mesocricetus auratus and the laboratory mouse mus musculus Zeitschrift fuer Versuchstierkunde 21(2): 61-71
Irmer, S.; Wissdore, H. 1978: Osteological studies on the vertebrae of the mongolian gerbil meriones unguiculatus in comparison with the thoracic and lumbar vertebrae of the syrian hamster mesocricetus auratus and the laboratory mouse mus musculus Zeitschrift fuer Versuchstierkunde 20(5): 241-255
Wissdorf, H.; Irmer, S. 1979: Anatomy of the pelvic and hindlimb bones of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867) as compared to those of the Syrian hamster, Mesocricetus auratus (Waterhouse, 1840) and the laboratory mouse, Mus musculus (Linné, 1758) Zeitschrift für Versuchstierkunde 21(3): 163-175
Irmer, S.; Wissdorf, H. 1980: Osteologic findings on the cranial bones in the mongolian gerbil meriones unguiculatus in comparison with the cranial bones of the syrian hamster mesocricetus auratus and those of the laboratory mouse mus musculus Zeitschrift fuer Versuchstierkunde 22(3): 179-191
Wissdorf, H.; Irmer, S. 1979: Osteological findings in the thorax of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867), compared to the thorax of the Syrian golden hamster, Mesocricetus auratus (Waterhouse, 1840), and to the laboratory mouse, Mus musculus (Linné, 1758) Zeitschrift für Versuchstierkunde 21(1): 17-20
Irmer, S.W.ssdorf, H. 1978: Osteological findings of vertebrates of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867), in comparison with the thorax and hip vertebrates of the Syrian golden hamster, Mesocricetus auratus (Waterhouse, 1840) and those of the laboratory mouse, Mus musculus (Linne, 1758) Zeitschrift fur Versuchstierkunde 5: 241-255
Irmer, S.; Wissdorf, H. 1979: Osteological findings in the skeleton of the shoulder of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867) compared to the shoulder of the Syrian golden hamster, Mesocricetus auratus (Waterhouse, 1840) and that of the laboratory mouse, Mus musculus (Linné, 1758) Zeitschrift für Versuchstierkunde 21(2): 61-71
Irmer, S.W.ssdorf, H. 1980: Osteological findings on the cranial bones of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867), compared with the facial bones of the Syrian golden hamster, Mesocricetus auratus (Waterhouse, 1840), and those of the laboratory mouse, Mus musculus (Linne, 1758) Zeitschrift fur Versuchstierkunde = Journal of Experimental Animal Science 2(5-6): 282-295
Irmer, S.; Wissdorf, H. 1980: Osteologic characteristics of the craniofacial bones of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867), compared with facial bones of the Syrian golden hamster, Mesocricetus auratus (Waterhouse, 1840), and those of the laboratory mouse, Mus musculus (Linné, 1758) Zeitschrift für Versuchstierkunde 22(5-6): 282-295
Irmer, S.; Wissdorf, H. 1980: Osteological studies of cranial bones of the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867), in comparison with cranial bones of the Syrian hamster, Mesocricetus auratus (Waterhouse, 1840), and those of the laboratory mouse, Mus musculus (Linné, 1758) Zeitschrift für Versuchstierkunde 22(3): 179-191
Irmer, S.W.ssdorf, H. 1980: Osteological discoveries on the cranial bones in the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867) in comparison with the cranial bones of the Syrian hamster, Mesocricetus auratus (Waterhouse, 1840) and that of the laboratory mouse, Mus musculus (Linne, 1758) Zeitschrift fur Versuchstierkunde 2(3): 179-191
Irmer, S.W.ssdorf, H. 1979: Osteological findings on the skeleton of the shoulder in the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867) in comparison to the skeleton of the shoulder of the golden Syrian hamster, Mesocricetus auratus (Waterhouse, 1840), and that of the laboratory mouse, Mus musculus (Linne, 1758) Zeitschrift fur Versuchstierkunde 1(2): 61-71
Wissdorf, H.I.mer, S. 1979: Osteological findings of the skeleton of the pelvic limbs in the Mongolian gerbil, Meriones unguiculatus (Milne-Edwards, 1867) in comparison to the skeleton of the pelvic limbs of the Syrian golden hamster, Mesocricetus auratus (Waterhouse, 1840) and the laboratory mouse, Mus musculus (Linne, 1758) Zeitschrift fur Versuchstierkunde 1(3): 163-176
Carraway, J.H.; Malone, J.B. 1985: Brugia pahangi: comparative susceptibility of the Mongolian jird, Meriones unguiculatus, and the PD4 inbred hamster, Mesocricetus auratus Experimental Parasitology 59(1): 68-73
De Oliveira, C.; Orsi, A.M.; Vicentini, C.A.; Duarte, H.E. 1993: Vascularização e drenagem venosa dos testículos e epidídimos no hamster (Mesocricetus auratus, W.) - Vascular patterns of the testes and epididymes in the syrian hamster (Mesocricetus auratus, W.) Naturalia (Sao Jose do Rio Preto) 18: 221-229
Reiter, R.J.; Johnson, L.Y.; Steger, R.W.; Richardson, B.A.; Petterborg, L.J. 1980: Pineal biosynthetic activity and neuro endocrine physiology in the aging hamster mesocricetus auratus and gerbil meriones unguiculatus Peptides 1(Suppl 1): 69-78
Hewing, M.; Bergmann, M. 1985: Differential permeability of pineal capillaries to lanthanum ion in the rat (Rattus norvegicus), gerbil (Meriones unguiculatus) and golden hamster (Mesocricetus auratus) Cell and Tissue Research 241(1): 149-154