+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Multiparameter geometric and densitometric analysis of the G0-G1 transition of WI-38 cells

Multiparameter geometric and densitometric analysis of the G0-G1 transition of WI-38 cells

Journal of Histochemistry and Cytochemistry 25(7): 724-729

Automated image analyses were performed using Feulgen stained smears of WI-38 cells that were either confluent, or that had received a nutritional stimulus to proliferate 3 hr before collection. These experiments show that it is possible to observe changes in morphometric and densitometric parameters of nuclei that correlate with structural and functional differences in isolated chromatins from quiescent G0 and proliferation G1 cells that have been demonstrated by other means. Scatter plot analyses of the data indicated the presence of nuclear images from the stimulated G1 population that had the same deoxyribonucleic acid content as the confluent G0 cells, but had greater areas, perimeters and horizontal projections and smaller mean free paths, form factors, and average optical densities. Multiparameter cluster analysis permits, even minimally, an objective, model-independent identification of G0 from G1 cells that present an increased nuclear dispersion (i.e., lower average optical density) systematically accompanied by increased nuclear convolution (i.e., lower form factor), both compatible with the reported increase in available binding sites with respect to G0 cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 038960053

Download citation: RISBibTeXText

PMID: 70453

DOI: 10.1177/25.7.70453

Related references

Multi parameter geometric and densitometric analyses of the g 0 to g 1 transition of wi 38 cells. Journal of Histochemistry & Cytochemistry 25(7): 724-729, 1977

The G0-G1 transition of WI38 cells. II. Geometric and densitometric texture analyses. Experimental Cell Research 106(1): 119-125, 1977

Experimental validation of geometric and densitometric coronary measurements on the new generation Cardiovascular Angiography Analysis System (CAAS II). Catheterization and Cardiovascular Diagnosis 30(2): 104-114, 1993

The analysis of densitometric and geometric parameters of bilateral proximal phalanges in horses with the use of peripheral quantitative computed tompgraphy. Acta Veterinaria Scandinavica 54: 41, 2012

Cell nucleus pattern analysis geometric and densitometric featuring automatic cell phase identification. Biology of the Cell 44(2): 177-188, 1982

Localization of the earlywood-latewood transition in oak rings using micro-densitometric analysis. Annales des Sciences Forestieres 55(4): 437-449, 1998

Extraction of prognostic and diagnostic information from multiparameter biochemical and cellular measurements by standardized multiparameter data analysis. Analytical Cellular Pathology 13(2): 98-99, 1997

Quantitative uv densitometric and fluoro densitometric analysis of mycophenolic acid on thin layer plates. Journal of Chromatography 207(3): 430-434, 1981

A method for the geometric and densitometric standardization of intraoral radiographs. Journal of Periodontology 54(7): 435-440, 1983

Geometric and densitometric standardization of intraoral radiography through use of a modified XCP system. Oral Surgery Oral Medicine Oral Pathology Oral Radiology & Endodontics 87(2): 253-257, 1999

Densitometric vs geometric quantitative angiography in the evaluation of ptca results. European Heart Journal 12(ABSTR Suppl.): 155, 1991

Measurement of the geometric and densitometric properties of the 2nd metacarpal by x ray and gamma ray densitometry. Physics in Medicine & Biology 16(3): 542, 1971

Correlation among geometric, densitometric, and mechanical properties in mandible and femur of osteoporotic rats. Journal of Bone and Mineral Metabolism 26(2): 130-137, 2008

Geometric-mathematical analysis of transition errors in the articulator and their practical effects. Deutsche Zahnarztliche Zeitschrift 32(8): 599-605, 1977

Multiparameter analysis and sorting of mammalian cells. Experimental Cell Research 84(1): 15-23, 1974