Section 39
Chapter 38,963

Nonspecific esterase activity in human lymphocytes. Histochemical characterization and distribution among major lymphocyte subclasses

Ranki, A.

Clinical Immunology and Immunopathology 10(1): 47-58


ISSN/ISBN: 0090-1229
PMID: 26491
DOI: 10.1016/0090-1229(78)90008-9
Accession: 038962224

Download citation:  

In a histochemical assay 75±8% of human blood lymphocytes hydrolyzed α-naphthyl acetate after an 8-hr exposition to the substrate. The brownish-red reaction product was easily distinguished in counterstained cytocentrifuged cell smears in lymphocyte cytoplasm. The reaction had a broad temperature optimum of 20°C, it was optimal at a p H of 5.8, and nearly all lymphocytes with histochemically demonstrable esterase activity were stained within 6 hr of exposure. Lipase activity was ruled out, as β-naphthyl acetate could also be used as substrate. Cholinesterases were also ruled out, as the reaction could not be inhibited by a 10 μM concentration of eserine. As the reaction was inhibited in 10 m M but not in 10 μM E-600, and could not be reactivated after E-600 inhibition with a 100 μM concentration of PCMB, the enzyme responsible for the reaction was defined as a nonspecific A-esterase. The α-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes were enriched and depleted by fractionation procedures which enriched and depleted the Srbc rosette-forming lymphocytes. The number of Anae marker-carrying lymphocytes closely followed the distribution of Srbc rosette-forming lymphocytes in spleen, tonsil, bone marrow, and blood and was inversely related to the number of surface Ig-carrying lymphocytes in these organs. Furthermore, visualization of the Anae marker simultaneously with the surface marker demonstrated that, while more than 80% of the Srbc rosette-forming lymphocytes carried the Anae marker, only 10%, at most, of the lymphocytes expressing large amounts of surface Ig were Anae positive. Discordant findings were observed in the thymus: 72% of the thymocytes formed rosettes with SRBC, whereas only 13% of the thymocytes carried the Anae marker. The results, therefore, suggest the presence of histochemically demonstrable nonspecific esterase activity predominantly in resting, mature T lymphocytes.

Full Text Article emailed within 0-6 h: $19.90