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Orientation of acidic polysaccharides and rhodopsin-oligosaccharides in frog retinal rod outer segments


, : Orientation of acidic polysaccharides and rhodopsin-oligosaccharides in frog retinal rod outer segments. Histochemistry 56(1): 65-77

Using topo-optical staining reactions, the presence and molecular order of three structural components of outer segments of frog retina were studied. These components included (1) an acidic polysaccharide texture, (2) free aldehyde groups which arise during formalin fixation and (3) the oligosaccharide chains of rhodopsin. Quantitative measurements of the dye binding and birefringence effects arising from the individual structural components in rod outer segments were made. Results indicated that all three structural components had a rather well-defined orientation within the ROS. The spherulites phagocytized from the apical ends of ROSs by the pigment epithelium also demonstrate preferred orientation of the three structural components investigated.

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Accession: 038964044

PMID: 77856

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Related references

Sineshchekov, V.A.; Balashov, S.P.; Litvin, F.F., 1983: Fluorescence of rhodopsin in the retinal rod outer segments of the frog at 77 K. Doklady Akademii Nauk SSSR 270(5): 1231-1235

Corless, J.M.; McCaslin, D.R.; Scott, B.L., 1982: Two-dimensional rhodopsin crystals from disk membranes of frog retinal rod outer segments. Two-dimensional crystals of rhodopsin have been prepared from purified frog disk membranes by using the detergent Tween 80. The space group of the orthorhombic crystals is p22121; the unit cell dimensions are 47 X 151 A. Projection maps of negativ...

de Turco, E.B.; Jackson, F.R.; Parkins, N.; Gordon, W.C., 2000: Strong association of unesterified [3H]docosahexaenoic acid and [3H-docosahexaenoyl]phosphatidate to rhodopsin during in vivo labeling of frog retinal rod outer segments. Docosahexaenoic acid (DHA, 22:6n-3), the most prevalent fatty acid in phospholipids of rod outer segments (ROS), is essential for visual transduction and daily renewal of ROS membranes. We investigated the association of [3H]DHA-lipids to rhodopsi...

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