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Osmometric studies of human immunoglobulin G

, : Osmometric studies of human immunoglobulin G. Journal of Immunological Methods 22(3-4): 319-325

Osmometric studies of solutions of IgG and its H and L chains were performed in various solvents. It has been found that in aqueous solutions and in guanidinium chloride solutions of low and moderate concentrations IgG molecules are associated which prevents molecular weight determination. On the other hand, in 6 M guanidinium chloride the molecular weight of IgG was determined to be 145,000. Addition of sodium dodecyl sulfate to aqueous solutions of IgG does not prevent association.

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