+ Translate

Outer-membrane penetration barriers as components of intrinsic resistance to beta-lactam and other antibiotics in Escherichia coli K-12

, : Outer-membrane penetration barriers as components of intrinsic resistance to beta-lactam and other antibiotics in Escherichia coli K-12. Antimicrobial Agents and ChemoTherapy 15(2): 182-189

A new technique has been devised to investigate the penetration of antibiotics through the gram-negative outer membrane; the application here was to study intrinsic resistance of Escherichia coli K-12. Exponential cells in broth were briefly treated with 2.5 mM ethylenediaminetetraacetic acid at 5 degrees C to disrupt the outer membrane penetration barrier, and the response of treated and untreated cells to antibiotics was compared by turbidimetry. A barrier index was derived to describe the ability of 7 beta-lactam and 10 other antibiotics to penetrate the outer membrane of strain Y10. There was correlation between the molecular weight and log(10) barrier index (r = 0.59, P congruent with 0.01). The envelope mutant D22 (envA) had low barrier indexes for erythromycin, rifampin, ampicillin, and cloxacillin. For the beta-lactams, outer membrane penetration and affinity for inner membrane target site(s) triggering cell lysis were measured as independent components of the overall activity; although penetration and overall activity varied greatly, the affinities of most were within a narrow range.

(PDF 0-2 workdays service)

Accession: 038964275

PMID: 106773

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Sawai T.; Matsuba K.; Yamagishi S., 1979: Role of outer membrane in the intrinsic resistance of gram negative bacteria to beta lactam antibiotics. Mitsuhashi, S (Ed ) Microbial Drug Resistance, Vol 2 2nd Symposium on Drug Resistance in Pathogenic Bacteria, Tokyo, Japan, Oct 26-28, 1977 Xii+439p Japan Scientific Societies Press: Tokyo, Japan; University Park Press: Baltimore, Md , Usa Illus : p345-348

Zimmermann, W.; Rosselet, A., 1977: Function of the outer membrane of Escherichia coli as a permeability barrier to beta-lactam antibiotics. On the basis of a simple theoretical model, the ease of penetration of beta-lactam antibiotics through the outer membrane of Escherichia coli was measured. The cell envelope was found to act as a diffusion barrier to both penicillins and cephalosp...

Leying H.J.; Karch K.; Kroll H.P.; Opferkuch W., 1985: The influence of beta lactam antibiotics including monobactam on the outer and inner membrane of escherichia coli. Adam, D , H Hahn And W Opferkuch (Ed ) The Influence Of Antibiotics on The Host-Parasite Relationship Ii Second International Symposium, Munich, West Germany, Mar 28-30, Xiv+298p Springer-Verlag: Berlin, West Germany; New York, N Y , Usa Illus 48-56

Leying, H.; Suerbaum, S.; Kroll, H.P.; Karch, H.; Opferkuch, W., 1986: Influence of beta-lactam antibiotics and ciprofloxacin on composition and immunogenicity of Escherichia coli outer membrane. The effects of subinhibitory concentrations of different beta-lactam antibiotics and one quinolone on the sedimentation of outer membranes (OMs) of Escherichia coli and on the qualitative properties and immunogenicity of OM components were studied...

Sawai, T.; Hiruma, R.; Kawana, N.; Kaneko, M.; Taniyasu, F.; Inami, A., 1982: Outer membrane permeation of beta-lactam antibiotics in Escherichia coli, Proteus mirabilis, and Enterobacter cloacae. Mutant strains lacking outer membrane protein(s) were isolated from Escherichia coli, Proteus mirabilis, and Enterobacter cloacae. The outer membrane protein(s) of P. mirabilis and E. cloacae corresponding to E. coli porin were identified on the b...

Nikaido, H.; Rosenberg, E.Y., 1990: Cir and Fiu proteins in the outer membrane of Escherichia coli catalyze transport of monomeric catechols: study with beta-lactam antibiotics containing catechol and analogous groups. Recently, beta-lactam agents containing iron-chelating moieties, such as E0702, which contains catechol, and pirazmonam and U-78,608, which contain 3-hydroxypyridone, have been developed. By determining the susceptibility to these agents of Escher...

Gryaznova N.S.; Subbotina N.A., 1989: Molecular organization of the outer membrane of bacteria and the penetration of beta lactam and aminoglycoside antibiotics through this membrane. Antibiotiki i Khimioterapiya 34(12): 925-932

Griaznova, N.S.; Subbotina, N.A., 1989: The molecular organization of the bacterial outer membrane and its penetration by antibiotics with beta-lactam and aminoglycoside structures. Antibiotiki i Khimioterapiia 34(12): 925-932

Scudamore, R.A.; Goldner, M., 1982: Penetration of the outer membrane of Pseudomonas aeruginosa by synergistic combinations of beta-lactam and aminoglycoside antibiotics. The mechanism of the synergistic action of carbenicillin-gentamicin and moxalactam-tobramycin combinations against Pseudomonas aeruginosa ATCC 9027 was investigated. Disruption of the outer membrane penetration barrier by EDTA treatment enhanced t...

Labedan, B., 1988: Increase in permeability of Escherichia coli outer membrane by local anesthetics and penetration of antibiotics. The MICs of several antibiotics (both hydrophobic and hydrophilic) which penetrate very poorly into intact Escherichia coli cells were found to be 2- to 10-fold decreased in the presence of low doses of various local anesthetics (procaine, dibucai...