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Oxidation of alpha-hydroxybutyrate by human serum

, : Oxidation of alpha-hydroxybutyrate by human serum. Clinica Chimica Acta; International Journal of Clinical Chemistry 81(3): 277-282

2-Hydroxybutyrate is enzymically oxidized by the LDH of human serum. Optimal concentrations of hydrogen ion, substrate and coenzyme for this oxidation by sera from patients with disease of the heart or liver have been determined at 37 degrees C. The linearity of this reaction is limited to a point at which an assay procedure employing a 2-hydroxyburyrate substrate seems unsuitable.

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Accession: 038964372

PMID: 21765

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Hagenfeldt, L.; Wahren, J., 1968: Human forearm muscle metabolism during exercise. 3. Uptake, release and oxidation of beta-hydroxybutyrate and observations on the beta-hydroxybutyrate/acetoacetate ratio. Scandinavian Journal of Clinical and Laboratory Investigation 21(4): 314-320

Lehninger, A.L.; Greville, G.D., 1953: The enzymic oxidation of alpha- and 2-beta-hydroxybutyrate. Biochimica et Biophysica Acta 12(1-2): 188-202

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Beyerinck, R.A.; Brass, E.P., 1987: Effects of alpha-ketobutyrate and alpha-hydroxybutyrate on the enzymatic determination of acetoacetate and beta-hydroxybutyrate. Clinical Chemistry 33(8): 1469-1470

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