+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Oxygen content determined by acrylamide polymerization: screening of anticancer agents, generation of oxyhemoglobin dissociation curves, and potential applications

Annals of Surgery 189(3): 275-282

Oxygen content determined by acrylamide polymerization: screening of anticancer agents, generation of oxyhemoglobin dissociation curves, and potential applications

Many investigators have endeavored to accurately measure oxygen content produced or consumed from substrates or tissues. Most of the techniques currently available are time consuming or exceedingly complex. Many require expensive apparatus and demand the skills of a highly trained technician. We have deveoloped a rapid and sensitive method of measuring oxygen content or consumption. This method is based upon the time for polymerization of acrylamide gels. The time of acrylamide polymerization is directly proportional to the oxygen content of the test medium. We have used this method to generate oxyhemoglobin dissociation curves which closely resemble those published by Severinghaus and Duvelleroy, as well as those we determined by the conventional Van Slyke analysis. We have also used this method to measure the oxygen consumption of tumor cells and to evaluate the effects of Actinomycin, cyclohexamide and cytosine arabinoside on Walker 256 ascites tumor. The results correlate with the known in vivo effects of these drugs on this tumor cell line. Therefore, because of its sensitivity and adaptability this method may be useful in a variety of clinical and biological situations.

(PDF 0-2 workdays service: $29.90)

Accession: 038964460

PMID: 85435

Related references

Oxygen consumption by acrylamide polymerization: a method for rapid screening of anticancer agents. Journal of Surgical Oncology 7(6): 513-520, 1975

Oxygen Content Determined by Acrylamide Polymerization. Annals of Surgery 189(3): 275-282, 1979

Oxygen consumption by acrylamide polymerization for rapid screening of chemotherapeutic agents. Surgical Forum 25(0): 87-89, 1974

Rapid method for determining effect of agents on oxyhemoglobin dissociation curves. Journal of Applied Physiology 28(3): 343-345, 1970

Determination of the oxygen capacity of the blood and oxyhemoglobin dissociation curves by polarographic coulometry. Bulletin of Experimental Biology and Medicine 74(3): 1120-1122, 1972

Determination of blood oxygen capacity and oxyhemoglobin dissociation curves by the method of polarographic coulometry. Biulleten' Eksperimental'noi Biologii i Meditsiny 73(9): 123-124, 1972

The Effect Of Chronic Oxygen Deficiency On Oxyhemoglobin Dissociation Curves In Successive Generations Of White Rats. Fiziologicheskii Zhurnal SSSR Imeni I. M. Sechenova 49: 615-620, 1963

Acrylamide polymerization: new method for determining the oxygen content in blood. Science 178(4057): 170-172, 1972

New method for oxygen content in blood based on acrylamide polymerization. Surgical Forum 23(0): 195-197, 1972

Hemoglobin oxygen affinity and adaptation to altitude: evidence for pre-adaptation to altitude in humans with left-shifted oxyhemoglobin dissociation curves. Transactions of the Association of American Physicians 91: 212-228, 1978