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Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography


Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography



Clinical Chemistry 22(9): 1497-1502



ISSN/ISBN: 0009-9147

PMID: 8221

DOI: 10.1093/clinchem/22.9.1497

Catecholamines and their metabolites have been separated isocratically by reverse-phase chromatography with aqueous (no organic solvent admixed) eluents. Unlike ion-exchange or ion-pair chromatography, mixtures of both acidic and basic substances can be separated in a single chromatographic run, because the retention is governed by hydrophobic interactions between the nonpolar moiety of the solute molecules and the octadecyl-silica stationary phase. The relative retention values strongly depend on the pH of the eluent, which governs the degree of dissociation of ionogenic solutes. The reproducibility of the results and the stability and efficiency of the chromatographic systems make this approach particularly attractive for use in clinical analysis.

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Accession: 038976481

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