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Spectral characteristics of metachromatically stained cartilage: effects of enzymatic degradation and dehydration

, : Spectral characteristics of metachromatically stained cartilage: effects of enzymatic degradation and dehydration. Acta Histochemica 58(2): 263-268

When a microspectrophotometer was used to study cartilage, stained metachromatically with Azure A and Safranin O, the following 3 points were made: 1. the absorption maximum of stained cartilage matrix is identical to that reported for solutions of these dyes containing chondroitin sulfate; 2. partial enzymatic degradation of the cartilage matrix has no effect on the position of the absorption peak (although the intensity is greatly diminished); 3. ethanolic dehydration shifts the absorption peak somewhat toward the orthochromatic position, and decreases the intensity of the stain.

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Accession: 038981054

PMID: 70941

DOI: 10.1016/S0065-1281(77)80135-9

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