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Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro



Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro



Biochemical Journal 158(1): 23-31



The amino acid-incorporating activities of free polyribosomes, rough membranes and rough membranes reconstituted in vitro, derived from rat liver, were compared. The amino acid-incorporating activity of the two membrane fractions were very similar in their response towards changes in pH, Mg2+ concentration and temperature, but differed from the response of the amino acid-incorporating activity of free polyribosomes. Free polyribosomes irreversibly lost part of their amino acid-incorporating capacity after they had become bound to rough membrane, from which the original ribosomes had been removed. Ribonuclease activity present in the membrane fraction may be responsible for this loss.

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Accession: 038982966

Download citation: RISBibTeXText

PMID: 9077

DOI: 10.1042/bj1580023


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