+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The preservation of ultrastructure in saturated phosphatidyl cholines by tannic acid in model systems and type II pneumocytes

, : The preservation of ultrastructure in saturated phosphatidyl cholines by tannic acid in model systems and type II pneumocytes. Journal of Cell Biology 74(3): 726-741

The preservation for electron microscopy of saturated phospholipids in general, and phosphatidyl choline (PC)in particular, remains and unsolved problem since OsO(4) and glutaraldehyde are incapable of interacting with PC directly. However, by introducing tannic acid preceding osmication, we were able to demonstrate highly ordered, preserved lamellar structures in model experiments with saturated PC, and in vivo experiments type II pneumocytes of lung tissue. The secretory bodies of the latter are known to contain a high proportion of these saturated phospholipids. In both cases, the repeating periodicity approximated 45 A. It was determined that tannic acid interacts with the choline component of PC to form a "complex," which then could be stabilized by treatment with OsO(4). In the absence of osmication, the PC-tannic acid complex acid did not survive conventional dehydration techniques, but osmication permitted conventional Epon embedment. Sphingomyelin (SPH), which contains choline, behaved similarly in model experiments. But there was no evidence of a comparable reaction with tannic acid using phosphatidyl ethanolamine (PEA), phosphatidyl serine (PS), or phosphstidy inositol (PI). Chemical studies indicted a high pH dependency for the formation of the PC- tannic acid complex. Also, experiments demonstrated its dissociation in various organic solvents. Sharp delineation and great contrast of the polar zones in the ordered lamellar structures was achieved by additional staining with lead citrate thus leading to the conclusion that tannic acid serves as a multivalent agent, capable of simultaneous interaction with saturated PC, OsO(4), and lead citrate stains.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 038991624

PMID: 71301

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Uchida, Hiroshi., 2001: Home range of a wintering young buzzard tracked by radio-transmitter. Strix, 19: 49-54

Tsialtas, J. T.; Pritsa, T. S.; Veresoglou, D. S., 2004: Leaf physiological traits and their importance for species success in a Mediterranean grassland. We related leaf physiological traits of four grassland species (Poa pratensis, Lolium perenne, Festuca valida, and Taraxacum officinale), dominant in a Mediterranean grassland, to their origin and success at community level. From early May to mid-...

Clarke P.B.S.; Hommer D.W.; Pert A.; Skirboll L.R., 1985: Electrophysiological actions of nicotine on substantia nigra single units. Extracellular recordings of single unit activity were made in the substantia nigra (SN) of chloral hydrate-anesthetized rats. Dopaminergic neurons of the pars compacta (SNC) were stimulated by (-)-nicotine bitartrate (1.0 mg/kg) given s.c. This ac...

Green, D. E., 1941: Hygiene in the war-time vegetable garden. The papers represented here are Nos. VII, VIII and IX of a series of articles dealing with diseases of more important vegetable crops and methods of control. The crops are as follows: - VII. Lettuce; VIII. Cucumbers and vegetable marrows; IX. Onio...

Kalwaryjski, Bernard Eug, 1928: Iodine storage and iodine binding by Tri-chinella. Pressed out preparations or 200[mu] thick frozen sections of trichinosed meat are left 1-2 hrs. in potassium iodide solution (iodine, 1 gm.; KI, 2 gm.; distilled water, 100 cc). Differentiate in 2.5% sodium thiosulphate, followed by thorough washi...

Berry, E.B., 1967: Treatment of gonorrheal urethritis evaluated in 230 men. During a recent 8-month deployment of an aircraft carrier to the western Pacific, 230 cases of acute gonorrheal urethritis were encountered. Patients were randomly assigned to one of 4 treatment schedules: procaine penicillin G for 10 days; procai...

Obata T.; Egashira T.; Yamanaka Y., 1989: Amine oxidase release into plasma of rats treated with hepatotoxin allyl formate. Amine oxidase activity in plasma of rats was investigated after pretreatment with the perilobular hepatotoxin allyl formate (AF). Amine oxidase activities in plasma elevated after administration of AF 0.1 ml/kg i.p. to male rats with 1 .mu.M and 1...

Maisuradze, Ni; Razmadze, Eg; Gurtskaia, Vg; Karaia, Rk, 1977: Varieties of grapefruit and Satsuma mandarin orange. Subtropicheskie kul'tury: 6) 137-145

Woloszyn, S; Rozmilowski, K; Orzechowska-Kulawczuk, M., 1982: Studies on a specific prevention against leptospirosis in pigs. Medycyna weterynaryjna 38(12): 641-644

Roback, SS., 1957: The immature tendipedids of the Philadelphia area (Diptera: Tendipedidae). Monographs Acad nat Sci Philad, 9, 1-151