+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A computer-assisted morphometric analysis of the organ of Corti

A computer-assisted morphometric analysis of the organ of Corti

Hearing Research 24(3): 189-201

This paper describes a method for a computer-assisted morphometric analysis of the organ of Corti and presents normal morphometric data on the chinchilla cochlea. The computer-assisted light microscope system that we developed consists of commercially available equipment: an Olympus light microscope, Boeckler X, Y motorized stage micrometers, an IBM PC computer and a computer program written by the second author. The system was developed for the analysis of whole-mount, surface preparations of the organ of Corti. The procedure for producing suitable surface preparations for a computer-assisted analysis of hair cells was described in a previous paper (Santi, 1986). The analysis procedure requires 2 passes along the length of the basilar membrane. During the first pass, the system establishes an X, Y slide coordinate system and maps basilar membrane distance onto the coordinate system. This procedure was performed in approximately 10 min using an efficient, 3-point arc approximation method. During the second pass, the user scans along the length of the basilar membrane and measures organ of Corti structures (e.g., hair cell dimensions) or records abnormal or missing hair cells. In addition to providing distance along the length of the basilar membrane, the system could also determine which hair cell row the user was examining. Thus, recording damaged and missing hair cells consisted of merely pressing the appropriate footpedal to indicate the type and degree of hair cell damage. After assessing hair cell damage, a cytocochleogram with an accompanying table of descriptive statistics was produced. Since the X,Y coordinate system for each slide was stored on a disk along with its corresponding basilar membrane distance map, slides could be removed and later reevaluated without the loss of previously obtained data. Normal morphometric data on the chinchilla organ of Corti consisted of cell dimensions within the reticular lamina and a two-dimensional graphical reconstruction of the curvature of the basilar membrane.

(PDF emailed within 0-6 h: $19.90)

Accession: 039053655

Download citation: RISBibTeXText

PMID: 3793637

DOI: 10.1016/0378-5955(86)90018-3

Related references

Organ of Corti surface preparations for computer-assisted morphometry. Hearing Research 24(3): 179-187, 1986

Cochlear changes from noise, kanamycin and ageing. I. Computer processing of histological data in the organ of Corti. II. Potentiating effects of noise and kanamycin. III. The organ of Corti in ageing guinea pigs. Laryngoscope 85(11 Pt 2 Suppl 1): 1-18, 1975

Computer-assisted morphometric analysis of the heart. Analytical and Quantitative Cytology and Histology 18(2): 129-136, 1996

Morphometric analysis of LPS-stimulated human monocytes by computer-assisted image analysis. Folia Biologica 46(3-4): 123-128, 1999

Morphometric analysis of pancreatic carcinoma by computer-assisted image analysis. Folia Biologica 46(1-2): 7-15, 1999

New analysis of hypertensive vascular changes recorded in ocular fundus photography using computer assisted morphometric analysis. Folia Ophthalmologica Japonica 36(10): 1972-1977, 1985

A new analysis of the hypertensive vascular change in ocular fundus photographs by using the computer assisted morphometric analysis. Nippon Ganka Gakkai Zasshi 89(11): 1261-1266, 1985

Computer assisted morphometric analysis of renal radiation injury. Analytical & Quantitative Cytology 4(2): 148, 1982

Computer-assisted morphometric analysis for three-dimensional cell shape. Laboratory Investigation; A Journal of Technical Methods and Pathology 41(4): 334-339, 1979

Computer assisted morphometric analysis for 3 dimensional cell shape. Laboratory Investigation 41(4): 334-339, 1979

Computer-assisted sperm analysis systems: morphometric aspects. Human Reproduction 10 Suppl 1: 46-52, 1995

Computer-assisted morphometric analysis of renal radiation response. Analytical and Quantitative Cytology 4(3): 174-180, 1982

Quantitative morphometric analysis of interface of esophageal squamous cell carcinoma , using a computer-assisted fractal analysis. Virchows Archiv 439(3): 279, September, 2001

Computer-assisted morphometric analysis of radiation injury in murine lymphocytes. Analytical and Quantitative Cytology 4(3): 181-187, 1982

Computer-assisted morphometric analysis of late renal radiation injury. Monographs in Clinical Cytology 9: 117-147, 1984