+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA



A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA



Journal of Biological Chemistry 250(11): 4060-4066



A deoxyribonuclease specific for methylated DNA was isolated from Diplococcus pneumoniae. The enzyme, an endonuclease, degrades DNA for Escherichia coli to fragments of average molecular weight about half a million; it forms discrete fragments from phage lambda DNA. Methyl-deficient E. coli DNA is not attacked, neither is DNA from Micrococcus radiodurans, which contains no methylated adenine or cytosine. Nor is DNA from D. pneumoniae or phage T7 attacked. However, DNA from M. radiodurans, D. pneumoniae, and T7 is attacked after methylation with and E. coli extract. Methylated T7 DNA is degraded to discrete fragments. Although the genetic transforming activity of normal DNA from D. pneumoniae is not affected by the enzyme, transforming activity of methylated DNA is destroyed. The enzyme is designated endonuclease R Dpn I. Under certain conditions another enzyme of complementary specificity can be isolated. This enzyme, designated endonuclease R Dpn II, produces a similar pattern of fragments from the DNA of T7 without prior methylation of the DNA. It also degrades normal DNA for D. pneumoniae. It is suggested that this pair of enzymes plays a role in some unknown control process, which would involve a large fraction of the specific base sequences that are methylated in E. coli DNA and are present but not methylated in DNA from other sources.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039058123

Download citation: RISBibTeXText

PMID: 236309


Related references

Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae. Proceedings of the National Academy of Sciences of the United States of America 71(6): 2305-2309, 1974

Identification of a deoxyribonuclease implicated in genetic transformation of Diplococcus pneumoniae. Journal of Bacteriology 123(1): 222-232, 1975

An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae. II. Evidence for its involvement in bacterial recombination. Biochimica et Biophysica Acta 224(1): 42-54, 1970

Membrane location of a deoxyribonuclease implicated in the genetic transformation of Diplococcus pneumoniae. Journal of Bacteriology 124(3): 1321-1329, 1975

An ATP-dependent deoxyribonuclease from diplococcus pneumoniae. I. Partial purification and some biochemical properties. Biochimica et Biophysica Acta 224(1): 29-41, 1970

Competence for deoxyribonucleic acid uptake and deoxyribonuclease action external to cells in the genetic transformation of Diplococcus pneumoniae. Journal of Bacteriology 114(1): 152-163, 1973

Adenosine triphosphate-dependent deoxyribonuclease from Diplococcus pneumoniae: fate of transforming deoxyribonucleic acid in a strain deficient in the enzymatic activity. Journal of Bacteriology 113(2): 718-723, 1973

The specific substance from diplococcus pneumoniae type 31. Carbohydrate Research 12(1): 89-96, 1970

The ultrastructure of the capsules of Diplococcus pneumoniae and Klebsiella pneumoniae stained with ruthenium red. Journal of General Microbiology 74(1): 21-31, 1973

Necrotizing myelitis a clinico pathologic report of 2 cases associated with diplococcus pneumoniae and mycoplasma pneumoniae infections. Trufant, Samuel A. Transactions Of The American Neurological Association Vol. 98. Montreal, Can., June 11-13,: New York, N.y., U.s.a. 290-295, 1973

Necrotizing myelitis: a clinico-pathologic report of two cases associated with diplococcus pneumoniae and mycoplasma pneumoniae infections. Transactions of the American Neurological Association 98: 290-292, 1973

Systematic purification of 5 glycosidases from streptococcus pneumoniae diplococcus pneumoniae. Journal of Biological Chemistry 252(23): 8615-8623, 1977

The specific substance from Diplococcus pneumoniae : Type 34 (u.s. type 41): the location of the O-acetyl groups. Carbohydrate Research 8(2): 214-218, 1968

The ultrastructure of encapsulated Diplococcus pneumoniae type 1 before and after exposure to type specific antibody. Laboratory Investigation; A Journal of Technical Methods and Pathology 15(4): 716-730, 1966

The specific substance from Diplococcus pneumoniae type 34 U. S. type 41). : Synthesis of methyl 3-O-β-d-galactofuranosyl-α-d-glucopyranoside. Carbohydrate Research 10(2): 213-219, 1969