Section 40
Chapter 39,101

A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography

Bahrami, S.; Gasser, H.; Redl, H.

Journal of Lipid Research 28(5): 596-598


ISSN/ISBN: 0022-2275
PMID: 3598402
Accession: 039100106

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We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The advantages of this method are the use of an isocratic solvent system limited to about 30 min and the very good separation of the phosphatidyl-choline fraction from the sphingomyelin fraction. Furthermore, the HPLC method has a better recovery rate than the thin-layer chromatography method, and it can be run under automatic control.

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