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A rapid method of preparing the (H3-H4-H2A-H2b)(2) histone octamer in large quantities



A rapid method of preparing the (H3-H4-H2A-H2b)(2) histone octamer in large quantities



Biokhimiia 56(10): 1864-1869



A simple and fast method for isolation of large amounts of the histone octamer (H2A-H2B-H3-H4)2 is proposed. This method is based on chromatin adsorption by hydroxyapatite with subsequent extraction of the histone octamer with 50 mM sodium-phosphate buffer containing 4 M NaCl pH 8.0. It was shown that the properties of the histone octamer isolated by this extractive procedure are identical with those of the histone octamer obtained by elution on a Sephadex G-100 column. The histone tetramer (H3-H4)2 and dimer (H2A-H2B) were obtained after gel filtration on Sephadex G-100 in 50 mM sodium-acetate (pH 5.6).

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Accession: 039106374

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PMID: 1777524


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