EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Agonist-sensitive calcium pool in the pancreatic acinar cell. II. Characterization of reloading


Agonist-sensitive calcium pool in the pancreatic acinar cell. II. Characterization of reloading



American Journal of Physiology 255(2 Part 1): G229-G235



ISSN/ISBN: 0002-9513

PMID: 3136660

DOI: 10.1152/ajpgi.1988.255.2.g229

45Ca2+ fluxes and free cytosolic Ca2+ measurements in guinea pig pancreatic acini indicated that after agonist stimulation and the release of Ca2+ from the agonist-sensitive pool at least part of the Ca2+ is extruded from the cell, resulting in 45Ca2+ efflux. In the continued presence of agonist, the pool remains permeable to Ca2+ but partially refills with Ca2+. This reloading is dependent on the concentration of extracellular Ca2+. In the absence of extracellular Ca2+, the pool is completely depleted of Ca2+. However, with increasing concentrations of CaCl2 in the incubation solution (from 0.5 to 2.0 mM) there is increasing repletion of the pool with Ca2+ during agonist stimulation. With termination of agonist stimulation, the Ca2+ permeability of the agonist-sensitive pool is rapidly reduced to that measured in the unstimulated cell. As a result, the Ca2+ incorporated into the pool during the stimulation period is rapidly trapped within the pool and exchanges poorly with medium Ca2+. Subsequently, the pool completely refills with Ca2+. The rate of Ca2+ reloading at the termination of agonist stimulation is slower than the conversion of the pool to the impermeable state. In incubation media containing 1.3 mM CaCl2, the half-time for reloading at the termination of stimulation is 5 min. These observations demonstrate the characteristics of Ca2+ reloading of the agonist-sensitive pool both during stimulation and at the termination of stimulation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039207497

Download citation: RISBibTeXText

Related references

Reloading of the agonist sensitive calcium pool in the pancreatic acinar cell. Gastroenterology 92(5 Part 2): 1543, 1987

Agonist-sensitive calcium pool in the pancreatic acinar cell. I. Permeability properties. American Journal of Physiology 255(2 Part 1): G221-G228, 1988

Th agonist sensitive pool of calcium regulates cellular cyclic gmp in the pancreatic acinar cell. Gastroenterology 102(4 Part 2): A284, 1992

Rapid desensitization of calcium release from the agonist sensitive pool in the pancreatic acinar cell. Gastroenterology 98(5 Part 2): A520, 1990

The agonist-sensitive calcium pool in the pancreatic acinar cell. Activation of plasma membrane Ca2+ influx mechanism. Journal of Biological Chemistry 262(35): 16963-16968, 1987

The agonist sensitivity calcium pool in the pancreatic acinar cell activation of plasma membrane calcium influx mechanism. Journal of Biological Chemistry 262(35): 16963-16968, 1987

Isolation of subcellular agonist-sensitive calcium stores from the pancreatic acinar cell. Cell Calcium 18(5): 364-376, 1995

Isolation of subcellular agonist-sensitive calcium stores in the pancreatic acinar cell. Gastroenterology 104(4 Suppl): A327, 1993

Co-expression of a dihydropyridine-sensitive calcium and a omega-conotoxin-sensitive calcium channel in a tumoral pancreatic acinar cell line. Gastroenterology 108(4 Suppl): A344, 1995

In situ imaging of agonist-sensitive calcium pools in AR4-2J pancreatoma cells. Evidence for an agonist- and inositol 1,4,5-trisphosphate-sensitive calcium pool in or closely associated with the nuclear envelope. Journal of Biological Chemistry 267(35): 25568-25575, 1992

Sphingosine regulates Ca(2+)-ATPase and reloading of intracellular Ca2+ stores in the pancreatic acinar cell. Biochimica et Biophysica Acta 1195(1): 45-50, 1994

Cellular mechanisms mediating agonist-stimulated calcium influx in the pancreatic acinar cell. Annals of the new York Academy of Sciences 713: 41-48, 1994

Characterization of inositol 1 4 5 trisphosphate sensitive and insensitive calcium pools in rat pancreatic acinar cells. FASEB Journal 3(3): A602, 1989

Physiological localization of an agonist-sensitive pool of Ca2+ in parotid acinar cells. Proceedings of the National Academy of Sciences of the United States of America 86(1): 167-171, 1989

Cyclic GMP mediates the agonist-stimulated increase in plasma membrane calcium entry in the pancreatic acinar cell. Journal of Biological Chemistry 265(22): 12846-12853, 1990