+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Alpha- and beta-adrenoceptor regulation of cyclic AMP accumulation in cultured rat astrocytes. A comparison of primary protoplasmic and mixed fibrous/protoplasmic astroglial cultures



Alpha- and beta-adrenoceptor regulation of cyclic AMP accumulation in cultured rat astrocytes. A comparison of primary protoplasmic and mixed fibrous/protoplasmic astroglial cultures



Biochemical Pharmacology 42(1): 59-69



The effect of noradrenaline and isoprenaline on cyclic AMP accumulation has been investigated in primary rat astrocytes which contain either (a) protoplasmic astrocytes alone or (b) both fibrous and protoplasmic astrocytes. Isoprenaline and noradrenaline stimulated cyclic AMP formation in both astrocyte culture preparations. Combinations of noradrenaline (1 microM) and isoprenaline (1 microM) produced a cyclic AMP response which was 58% and 26% of that produced by isoprenaline alone in protoplasmic and mixed fibrous/protoplasmic cultures, respectively. In both preparations this inhibitory effect of noradrenaline was antagonized by the alpha 2-adrenoceptor antagonist yohimbine (1 microM). A striking feature of the concentration-response curve for isoprenaline (EC50 = 0.8 microM) in mixed fibrous/protoplasmic cultures was that the cyclic AMP response decreased sharply at concentrations above 1 microM. This phenomenon was not seen in cultures containing protoplasmic astroglia alone. The fall in the isoprenaline concentration-response curve was not observed in the presence of the alpha-adrenoceptor antagonist phentolamine (1 microM), the dihydropyridine calcium antagonist isradipine (10 microM), the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.1 mM) or in nominally calcium-free medium. The effect of phentolamine was mimicked by the alpha 1-adrenoceptor antagonist prazosin (1 microM) but not by the alpha 2-antagonist yohimbine (1 microM). In conclusion, the data from this study suggest that two different populations of astrocytes in in vitro culture are able to raise intracellular cyclic AMP levels via beta-adrenoceptor activation and that there are differences in the extent of alpha-adrenoceptor (both alpha 1- and alpha 2-) mediated inhibition of cyclic AMP accumulation between the two primary astroglial cell preparations.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039218474

Download citation: RISBibTeXText

PMID: 1648923


Related references

Alpha adrenoceptor and beta adrenoceptor regulation of cyclic amp accumulation in cultured rat astrocytes a comparison of primary protoplasmic and mixed fibrous protoplasmic astroglial cultures. Biochemical Pharmacology 42(1): 59-70, 1991

Reactive protoplasmic and fibrous astrocytes contain high levels of calpain-cleaved alpha 2 spectrin. Experimental and Molecular Pathology 100(1): 1-7, 2016

Melatonin inhibits beta-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures. Neuroendocrinology 38(3): 176-181, 1984

Extensive fibrillar protoplasmic differentiations and their significance for protoplasmic streaming. VII. Experimental induction, contraction and extraction of the protoplasmic fibrils of Physarum polycephalum. Zeitschrift für Zellforschung und Mikroskopische Anatomie 109(3): 420-430, 1970

Extensive fibrillar protoplasmic differentiations and their significance for protoplasmic streaming part 7 experimental induction contraction and extraction of the protoplasmic fibrils of physarum polycephalum. Zeitschrift fuer Zellforschung und Mikroskopische Anatomie 109(3): 420-430, 1970

Extensive fibrillar protoplasmic differentiations and their significance for protoplasmic streaming. X. The arrangement of actomyosin-fibrils in experimentally unaffected protoplasmic veins of Physarum in situ. Protistologica 1 (1) 19-30, 1975

Extensive fibrillar protoplasmic differentiations and their significance for protoplasmic streaming part 10 the arrangement of acto myosin fibrils in experimentally unaffected protoplasmic veins of physarum polycephalum in situ. Protistologica 11(1): 19-30, 1975

Expression of gamma-glutamyl transpeptidase in mouse perivascular astrocytes and in a protoplasmic-like astroglial cell clone. Brain Research 852(1): 191-197, 2000

Fibrous and protoplasmic astrocytes are biochemically and developmentally distinct. Journal of Neuroscience 4(2): 585-592, 1984

Adenylyl cyclase activation underlies intracellular cyclic AMP accumulation, cyclic AMP transport, and extracellular adenosine accumulation evoked by beta-adrenergic receptor stimulation in mixed cultures of neurons and astrocytes derived from rat cerebral cortex. Brain Research 692(1-2): 227-232, 1995

Preferential histochemical staining of protoplasmic and fibrous astrocytes in rat CNS with GFAP antibodies using different fixatives. Brain Research 518(1-2): 347-352, 1990

Fibrous and protoplasmic astrocytes express GABA-A receptors that differ in benzodiazepine pharmacology. Brain Research. 636(1): 73-80, 1994

Investigation of mGlu receptors in protoplasmic and fibrous astrocytes Implications for receptor cross-talk. British Journal of Pharmacology 133(Proceedings Suppl.): 141P, 2001

Melatonin Inhibits -Adrenoceptor-Stimulated Cyclic Amp Accumulation in Rat Astroglial Cell Cultures. Neuroendocrinology 38(3): 176-181, 1984

Adenosine-dependent regulation of cyclic AMP accumulation in primary cultures of rat astrocytes and neurons. Journal of Neuroscience Research 30(4): 631-640, 1991