+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Alpha-Glutamyl-beta-naphthylamide hydrolase of rabbit small intestine. Localization in the brush border and separation from other brush border peptidases

Alpha-Glutamyl-beta-naphthylamide hydrolase of rabbit small intestine. Localization in the brush border and separation from other brush border peptidases

Biochimica et Biophysica Acta 419(1): 42-50

About 70% of the total mucosal enzymatic activity hydrolyzing beta-L-glutamyl-beta-naphthylamide in the rabbit small intestine is present in the brush border; the specific activity in this subcellular fraction is 7 times higher than that of the homogenate. Similar results are obtained for L-leucyl beta-naphthylamide hydrolase. The enzyme activity is efficiently solubilized by papain digestion and is clearly separated from L-leucyl-beta-naphthylamide hydrolase by chromatography on concanavalin A-Sepharose. It probably represents a digestive peptidase, different from the other known peptide hydrolases of the digestive surface of the small intestine.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 039218757

Download citation: RISBibTeXText

PMID: 1244859

Related references

Purification and partial characterization of a retinyl ester hydrolase from the brush border of rat small intestine mucosa: Probable identity with brush border phospholipase B. Biochemistry 33(9): 2661-2666, 1994

An in vitro model for the analysis of intestinal brush border assembly. II. Changes in expression and localization of brush border proteins during cell contact-induced brush border assembly in Caco-2BBe cells. Journal of Cell Science 105: 461-472, 1993

Brush border peptidases of rabbit and human intestine. Acta Paediatrica Belgica 31(3): 172, 1978

The distribution of brush border peptidases along the small intestine of the adult human. Pediatric Research 15(5): 884-885, 1981

The localization of peptidases in microvilli from the brush border of the proximal renal tubule of the rabbit. Biochemical Journal 115(3): 18p, 1969

Peptidases of brush border of human and rabbit small intestinal mucosa. Pediatric Research 10(10): 879, 1976

Development of brush border peptidases in human and rat small intestine during fetal and neonatal life. Pediatric Research 15(7): 991-995, 1981

Distribution of brush-border membrane peptidases along the rabbit intestine: implication for oral delivery of peptide drugs. Life Sciences 52(11): 941-947, 1993

Epidermal growth factor stimulates ileal brush border Na/H exchange by stimulation of a brush border non-receptor tyrosine kinase and increases brush border PI-3 kinase. Gastroenterology 106(4 Suppl. ): A243, 1994

Characteristics of the microvillus brush border of human placenta: insulin receptor localization in brush border membranes. Endocrinology 103(4): 1458-1468, 1978

A retinyl ester hydrolase activity intrinsic to the brush border membrane of rat small intestine. Biochemistry 31(11): 2920-2926, 1992

Effects of diets on hydrolase activities localized in the brush border membrane of the small intestine in rats. Agricultural & Biological Chemistry 53(9): 2489-2496, 1989

Developmental regulation of brush border hydrolase and iron transporter gene expression in pig small intestine. Journal of Dairy Science 88: 296-296, 2005

Immunoelectrophoretic studies on human small intestinal brush border proteins: cellular alterations in the levels of brush border enzymes after jejunoileal bypass operation. Gut 21(8): 662-668, 1980

Binding of soybean agglutinin to small intestinal brush border membranes and brush border membrane enzyme activities in Atlantic salmon (Salmo salar). Aquaculture 91(1/2): 163-170, 1990