+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Alterations in hepatic delta-aminolevulinic acid synthetase and heme oxygenase activities after chronic ethanol consumption in rats



Alterations in hepatic delta-aminolevulinic acid synthetase and heme oxygenase activities after chronic ethanol consumption in rats



Alcohol 8(6): 449-451



In the present study, the effect of chronic ethanol consumption in rats on the hepatic heme metabolism was investigated. Male Wistar rats were fed a nutritionally adequate liquid diet containing ethanol as 36% of the total calories for 5 weeks. After an overnight fast, the livers were excised and centrifuged to obtain mitochondrial and microsomal fractions. Chronic ethanol feeding of rats resulted in about 19% hepatomegaly as represented by the increased liver/body weight ratio. There was no difference in the mitochondrial protein content between the ethanol-treated and control rats, but the microsomal protein content was significantly increased in the ethanol-treated rats. Hepatic microsomal content of cytochrome P-450 (P-450) was markedly enhanced by chronic ethanol ingestion. Microsomal contents of cytochrome b5 (b5) and total heme were also increased to a lesser extent. After chronic ethanol abuse, the hepatic activity of delta-aminolevulinic acid (ALA) synthetase, which is a rate-limiting enzyme for heme production, was significantly increased and that of the heme oxygenase was slightly increased. These data indicate that ALA synthetase activity is induced by the negative feedback mechanism in order to compensate the depletion of heme caused by the utilization of heme for P-450. It is also speculated that, in response to excessive production of heme as described above, heme oxygenase activity is secondarily induced to regulate the amount of heme.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039221166

Download citation: RISBibTeXText

PMID: 1781921


Related references

Regulation of rat hepatic delta-aminolevulinic acid synthetase and heme oxygenase activities: evidence for control by heme and against mediation by prosthetic iron. International Journal of Biochemistry 15(4): 479-485, 1983

Alterations of hepatic delta aminolevulinic acid synthetase heme oxygenase microsomal cytochrome content and drug metabolism in rats bearing ascitic tumors ah 13 ah 66 and ah 414 and a 3 methylcholanthrene induced tumor. Journal of Pharmacobio-Dynamics 7(7): 501-510, 1984

Cyclic oscillations in rat hepatic heme oxygenase and delta-aminolevulinic acid synthetase following intravenous heme administration. Archives of Biochemistry and Biophysics 173(1): 11-17, 1976

Cadmium induced alterations in the activities of hepatic delta amino levulinic acid synthetase and heme oxygenase in mice. Journal of Pharmacobio-Dynamics 2(2): 84-91, 1979

Effects of perphenazine, chlorpromazine or CoCl2 on the activities of delta-aminolevulinic acid synthetase and heme oxygenase and on the content of hemoprotein in rat liver. Research Communications in Chemical Pathology and Pharmacology 65(1): 57-64, 1989

Delta-Aminolevulinic acid synthetase and heme oxygenase activity in lung and liver of rats given DDT and endosulfan intratracheally. Bulletin of Environmental Contamination and Toxicology 34(1): 24-28, 1985

Effects of perphenazine chlorpromazine or cobaltous chloride on the activities of delta aminolevulinic acid synthetase and heme oxygenase and on the content of hemoprotein in rat liver. Research Communications in Chemical Pathology & Pharmacology 65(1): 57-64, 1989

Regulation of rat hepatic delta amino levulinic acid synthetase ec 2.3.1.37 and heme oxygenase ec 1.14.99.3 activities evidence for control by heme and against mediation by prosthetic iron. International Journal of Biochemistry 15(4): 479-486, 1983

Changes in the male rat hepatic cytochrome P-450 level, heme oxygenase and delta-aminolevulinic acid synthase activities at various stages of life. Mechanisms of Ageing and Development 74(1-2): 79-88, 1994

Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on heme oxygenase-1, biliverdin IXalpha reductase and delta-aminolevulinic acid synthetase 1 in rats with wild-type or variant AH receptor. Toxicology 250(2-3): 132-142, 2008

Acute effect of amitriptyline, phenobarbital or cobaltous chloride on delta-aminolevulinic acid synthetase, heme oxygenase and microsomal heme content and drug metabolism in rat liver. Japanese Journal of Pharmacology 50(3): 289-293, 1989

Coordinate up-regulation of CYP1A1 and heme oxygenase-1 (HO-1) expression and modulation of delta-aminolevulinic acid synthase and tryptophan pyrrolase activities in pyridine-treated rats. Biochemical Pharmacology 58(4): 723-734, 1999

Effects of chlorinated benzenes on the activities of delta amino levulinic acid synthetase and heme oxygenase and on the content of hemo protein in the liver of rats. Journal of Pharmacobio-Dynamics 4(1): 69-76, 1981