+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Altered regulation of glycogen metabolism by vasopressin and phenylephrine in hepatocytes from insulin-resistant obese (fa/fa) rats. Role of protein kinase C

Altered regulation of glycogen metabolism by vasopressin and phenylephrine in hepatocytes from insulin-resistant obese (fa/fa) rats. Role of protein kinase C

Biochemical Journal 269(3): 795-799

The hormonal control of glycogen synthase and phosphorylase interconversion was investigated in hepatocytes isolated from lean and genetically obese (fa/fa) rats. In cells from obese animals, the inactivation of synthase by 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA), phospholipase C, vasopressin and the alpha 1-adrenergic agonist phenylephrine was markedly impaired, and the property of PMA to counteract phosphorylase activation by phenylephrine was attenuated. The maximal response of phosphorylase activation to phenylephrine and vasopressin was increased in obese-rat hepatocytes, but the sensitivity to these hormones was similar to that in lean-rat hepatocytes. These observations indicate that the defect in protein kinase C that we reported previously in heart of insulin-resistant fa/fa rats [van de Werve, Zaninetti, Lang, Vallotton & Jeanrenaud (1987) Diabetes 36, 310-319] is probably also expressed in liver.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 039223449

Download citation: RISBibTeXText

PMID: 2117921

DOI: 10.1042/bj2690795

Related references

Protein kinase CK2 is altered in insulin-resistant genetically obese (fa/fa) rats. FEBS Letters 437(3): 211-215, 1998

Chronic activation of protein kinase C in soleus muscles and other tissues of insulin-resistant type II diabetic Goto-Kakizaki (GK), obese/aged, and obese/Zucker rats. A mechanism for inhibiting glycogen synthesis. Diabetes 45(10): 1396-1404, 1996

Fasting enhances glycogen synthase activation in hepatocytes from insulin-resistant genetically obese (fa/fa) rats. Biochemical Journal 269(3): 789-794, 1990

Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa/fa rats. Febs Journal 273(9): 1989-1999, 2006

The role of acetyl CoA carboxylase and 5 AMP-activated protein kinase in the altered metabolism of insulin resistant rat hearts. Circulation 94(8 Suppl. ): I125, 1996

Insulin stimulation of mitogen-activated protein kinase, p90rsk, and p70 S6 kinase in skeletal muscle of normal and insulin-resistant mice. Implications for the regulation of glycogen synthase. Journal of Biological Chemistry 270(50): 29928-29935, 1995

Postexercise muscle triacylglycerol and glycogen metabolism in obese insulin-resistant Zucker rats. Obesity Research 12(7): 1158-1165, 2004

Role of pectin from cucumber (Cucumis sativus) in modulation of protein kinase C activity and regulation of glycogen metabolism in rats. Indian Journal of Biochemistry & Biophysics 44(3): 183-185, 2007

Differential effects of vasopressin and phenylephrine on protein kinase c mediated protein phosphorylations in isolated hepatocytes. Federation Proceedings 45(6): 1860, 1986

Prevalent role of the insulin receptor isoform A in the regulation of hepatic glycogen metabolism in hepatocytes and in mice. Diabetologia 59(12): 2702-2710, 2016

Adenovirus-mediated transfer of the muscle glycogen phosphorylase gene into hepatocytes confers altered regulation of glycogen metabolism. Journal of Biological Chemistry 267(35): 25129-25134, 1992

New emerging role of protein-tyrosine phosphatase 1B in the regulation of glycogen metabolism in basal and TNF-α-induced insulin-resistant conditions in an immortalised muscle cell line isolated from mice. Diabetologia 54(5): 1157-1168, 2011

Regulation of glycogen metabolism in primary cultures of rat hepatocytes. Restoration of acute effects of insulin and glucose in cells from diabetic rats. Journal of Biological Chemistry 261(2): 785-790, 1986

Glycogen Metabolism and Rheumatoid Arthritis: The Role of Glycogen Synthase 1 in Regulation of Synovial Inflammation via Blocking AMP-Activated Protein Kinase Activation. Frontiers in Immunology 9: 1714, 2018

Effects of vasopressin on fructose and glycogen metabolism in hepatocytes from fed and fasted rats. Archives of Biochemistry & Biophysics 212(1): 43-53, 1981