Section 40
Chapter 39,231

Amino acid sequence of heat-stable enterotoxin produced by Vibrio cholerae non-01

Takao, T.; Shimonishi, Y.; Kobayashi, M.; Nishimura, O.; Arita, M.; Takeda, T.; Honda, T.; Miwatani, T.

Febs Letters 193(2): 250-254


ISSN/ISBN: 0014-5793
PMID: 4065341
DOI: 10.1016/0014-5793(85)80163-0
Accession: 039230248

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The amino acid sequence of heat-stable enterotoxin, produced by Vibrio cholerae non-01 and isolated from its culture supernatant, was determined by both Edman degradation of native and reductively carboxymethylated enterotoxin and also a combination of fast atom bombardment mass spectrometry and carboxypeptidase Y digestion of native enterotoxin to be as follows: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This sequence is very similar, but not identical, to those of heat-stable enterotoxins produced by enterotoxigenic Escherichia coli and Yersinia enterocolitica.

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