EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Antagonizing effects of phorbol 12-myristate 13-acetate on hormonally stimulated gluconeogenesis in isolated rat hepatocytes involve activity changes of pyruvate kinase


Antagonizing effects of phorbol 12-myristate 13-acetate on hormonally stimulated gluconeogenesis in isolated rat hepatocytes involve activity changes of pyruvate kinase



Archives of Biochemistry and Biophysics 281(2): 185-190



ISSN/ISBN: 0003-9861

PMID: 2168154

DOI: 10.1016/0003-9861(90)90430-7

The tumor-promoting phorbol ester phorbol 12-myristate 13-acetate partially neutralized the stimulatory effects of epinephrine (alpha 1-adrenergic actions), glucagon, and dibutyryl-cAMP on gluconeogenesis in isolated hepatocytes of fasted rats, when lactate or dihydroxyacetone was used as the substrate. By constructing metabolic crossover plots and by comparing rates of lactate production from dihydroxyacetone with K0.5 values of extracted pyruvate kinase for phosphoenolpyruvate, we obtained evidence that phorbol ester actions on hormonally stimulated gluconeogenesis were accompanied by proportionate increases in activity of pyruvate kinase. Although purified pyruvate kinase from rat liver was a substrate for protein kinase C in vitro, phosphorylation was not accompanied by modulation of kinetic parameters. Furthermore, incubation of pyruvate kinase extracted from hormone-treated hepatocytes with protein kinase C revealed no activation of the prephosphorylated enzyme. This and the absence of effects of the phorbol ester on basal rates of gluconeogenesis and lactate production suggest that effects of protein kinase C on pyruvate kinase activity in hepatocytes may result from impairment of steps at the level of hormone-induced signal transduction.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039286122

Download citation: RISBibTeXText

Related references

Insulin regulation of pyruvate kinase activity in cultured rat hepatocytes, in the presence of vasopressin, ionophore A23187 or 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. Biochemical Journal 252(3): 717-721, 1988

Hormone-induced changes in pyruvate kinase activity in isolated hepatocytes II. Relation to the hormonal regulation of gluconeogenesis gluconeogenesis. Biochimica et Biophysica Acta - General Subjects 500(2): 267-276, 1977

Protein kinase-C activity in phorbol myristate acetate-stimulated neutrophils from newborn and adult cattle. American Journal of Veterinary Research 53(9): 1679-1684, 1992

Prostanoid production in the phorbol myristate acetate stimulated isolated perfused rabbit lung effect of protein kinase c inhibition. Clinical Research 35(3): 536A, 1987

The Hormonal Control of Pyruvate Kinase Activity and of Gluconeogenesis in Isolated Hepatocytes. Biochemical Society Transactions 4(6): 1048.1-1048, 1976

Hormonal control of pyruvate kinase activity and of gluconeogenesis in isolated hepatocytes. Proceedings of the National Academy of Sciences of the United States of America 73(8): 2762-2766, 1976

Hormonal modulation of c-fos expression in isolated hepatocytes. Effects of angiotensin II and phorbol myristate acetate on transcription and mRNA degradation. Biochimica et Biophysica Acta 1310(2): 217-222, 1996

Effects of adenosine in low dose phorbol myristate acetate pma stimulated neutrophil injury in isolated rabbit lungs. American Review of Respiratory Disease 139(4 Part 2): A300, 1989

Inhibition of NF-IL6 activity by manassantin B, a dilignan isolated from Saururus chinensis, in phorbol myristate acetate-stimulated U937 promonocytic cells. Molecules and Cells 20(1): 105-111, 2005

Effect of dexamethasone on gluconeogenesis, pyruvate kinase, pyruvate carboxylase and pyruvate dehydrogenase flux in isolated hepatocytes. Biochemical Journal 289: 821-828, 1993

Toxicity of phorbol myristate acetate-stimulated polymorphonuclear neutrophils against rat hepatocytes. Demonstration and mechanism. Laboratory Investigation; a Journal of Technical Methods and Pathology 59(6): 831-837, 1988

Effects of phorbol 12 13 dibutyrate and phorbol 12 myristate 13 acetate pma on the alpha 1 adrenergic signaling process in hepatocytes. Journal of Cell Biology 107(6 Part 3): 66A, 1988

Phorbol myristate acetate stimulated polymorphonuclears toxicity against hepatocytes evidence for a protease mediated mechanism. Hepatology: 1210, 1986

Hormone-induced changes in pyruvate kinase activity in isolated hepatocytes. II. Relation to the hormonal regulation of gluconeogenesis. Biochimica et Biophysica Acta 500(2): 267-276, 1977

Identification of phorbol myristate acetate stimulated kinase in Zea mays. Journal of Plant Biochemistry and Biotechnology 5(1): 7-11, 1996