+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antibody against avian müllerian inhibiting substance (MIS) recognizes MIS on rat müllerian duct and human tumor cells



Antibody against avian müllerian inhibiting substance (MIS) recognizes MIS on rat müllerian duct and human tumor cells



Proceedings of the National Science Council Republic of China. Part B Life Sciences 13(4): 267-275



Müllerian inhibiting substance (MIS) on rat Müllerian duct (Md), and four Müllerian-derived tumor cells: HeLa S-3, RL-95.2, A-431 and NIH:OVCAR-3 are recognized by the poly- and mono-clonal avian-MIS-antibodies (A-MIS-Abs) using avidin-biotin complex (ABC) immunolabeling techniques. Internalization of MIS-ligand complexes was successfully detected in HeLa S-3, OVCAR-3 and RL 95.2 cells. Control groups include: (i) the samples omitted primary antibody treatment, (ii) Wolffian duct by side of Md in the genital ridge, and (iii) another two MIS-negative tumor cell lines of non-Müllerian origin: Chang hepatoma ascites cell and mouse myeloma cell (X63-Ag 8.653). Genital ridges from rat embryos of 14d of gestation were removed under dissection microscope, fixed in 2.5% glutaraldehyde in D-PBS of pH 7.2 for 30 min, and sliced into 0.1-0.2 mm thick pieces. A-431, HeLa S-3 and NIH:OVCAR-3 were maintained in OPTI-MEM culture medium, RL-95.2 was cultured in F12 culture medium. The cells were transferred to 24-well flat bottom culture plates with Thermanox tissue culture coverslips. The immunolabeling of fixed and non-fixed samples were processed within the wells. These studies provide first immunocytochemical evidences for the similarity between A-MIS and M-MIS molecules by polyclonal and monoclonal A-MIS-Ab. It has also proved that the tumor cell lines, which were subjects of MIS inhibition of cell growth, showed MIS binding on cell surfaces.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039295219

Download citation: RISBibTeXText

PMID: 2484046


Related references

Induction of WNT inhibitory factor 1 expression by Müllerian inhibiting substance/antiMullerian hormone in the Müllerian duct mesenchyme is linked to Müllerian duct regression. Developmental Biology 386(1): 227-236, 2014

Effect of human recombinant mullerian inhibiting substance on isolated epithelial and mesenchymal cells during mullerian duct regression in the rat. Endocrinology 131(3): 1481-1488, 1992

Insensitivity of the chick embryo müllerian duct to human recombinant müllerian-inhibiting substance. Journal of Molecular Endocrinology 8(1): 73-77, 1992

Insensitivity of the chick embryo Mullerian duct to human recombinant Mullerian-inhibiting substance. Journal of Molecular Endocrinology 8(1): 73-77, 1992

Antibody against avian muellerian inhibiting substance mis recognizes mis on rat muellerian duct and human tumor cells. Proceedings of the National Science Council Republic of China Part B Life Sciences 13(4): 267-275, 1989

Müllerian inhibiting substance regulates its receptor/SMAD signaling and causes mesenchymal transition of the coelomic epithelial cells early in Müllerian duct regression. Development 133(12): 2359-2369, 2006

Programmed cell death in the Müllerian duct induced by Müllerian inhibiting substance. American Journal of Anatomy 149(3): 353-375, 1977

Mullerian inhibiting substance binds to fetal lung and mullerian duct. Pediatric Research 31(4 Part 2): 40A, 1992

Müllerian inhibiting substance is present in testes of dogs with persistent müllerian duct syndrome. Biology of Reproduction 41(5): 881-888, 1989

Mullerian inhibiting substance is present in testes of dogs with persistent Mullerian duct syndrome. Biology of Reproduction 41(5): 881-889, 1989

Mullerian inhibiting substance is present in embryonic testes of dogs with persistent Mullerian duct syndrome. Biology of reproduction 48(6): 1410-1418, 1993

Expression of Müllerian-Inhibiting Substance/Anti-Müllerian Hormone Type II Receptor in the Human Theca Cells. Journal of Clinical Endocrinology and Metabolism 103(9): 3376-3385, 2018

Interspecific character of the Müllerian-inhibiting substance: action of the human fetal testis, ovary and adrenal of the fetal rat Müllerian duct in organ culture. Journal of Clinical Endocrinology and Metabolism 32(3): 404-409, 1971

Involution of the female Mullerian duct of the fetal rat in the organ-culture assay for the detection of Mullerian Inhibiting Substance. American Journal of Anatomy 156(2): 265-284, 1979

Mullerian duct regression and antiproliferative bioactivities of mullerian inhibiting substance reside in its carboxy-terminal domain. Endocrinology 131(1): 291-296, 1992