EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Application of thermospray liquid chromatography-mass spectrometry to the simultaneous quantification of tracer concentrations of isotopically labelled carbamazepine epoxide and steady-state levels of carbamazepine and carbamazepine epoxide



Application of thermospray liquid chromatography-mass spectrometry to the simultaneous quantification of tracer concentrations of isotopically labelled carbamazepine epoxide and steady-state levels of carbamazepine and carbamazepine epoxide



Journal of Chromatography 474(1): 223-230



A thermospray high-performance liquid chromatography-mass spectrometry method for the separation and quantification of tracer concentrations of isotopically labelled carbamazepine epoxide ([15N, 13C]CBZE) in the presence of steady-state levels of the anticonvulsant carbamazepine (CBZ) and its epoxide metabolite (CBZE) has been developed. The technique does not require derivatization, demonstrates little or no thermal degradation of the analytes, provides increased specificity not available from conventional high-performance liquid chromatography, and has a detection limit of 500 pg for CBZE on-column. The method, incorporating d4-CBZ and d4-CBZE as internal standards, allows precise and accurate determination of the analytes with good reproducibility and stability.

(PDF same-day service: $19.90)

Accession: 039313810

Download citation: RISBibTeXText

PMID: 2768394

DOI: 10.1016/s0021-9673(01)93917-2



Related references

Relationships between carbamazepine-diol, carbamazepine-epoxide, and carbamazepine total and free steady-state concentrations in epileptic patients: the influence of age, sex, and comedication. Therapeutic Drug Monitoring 18(6): 660-665, 1996

Simultaneous analysis of carbamazepine and carbamazepine 10 11 epoxide by gas chromatography chemical ionization mass spectrometry stable isotope methodology. Analytical Letters 11(2): 119-134, 1978

Determination of carbamazepine and carbamazepine 10,11-epoxide in human plasma by tandem liquid chromatography-mass spectrometry with electrospray ionisation. Journal of Chromatography B 769(1): 1-7, 25 March, 2002

Effect of Carbamazepine 10, 11-Epoxide on Serum Carbamazepine Measurement Using a New CMIA Assay: Comparison of Values Obtained by Using PETINIA, CEDIA and Liquid Chromatography Combined with Tandem Mass Spectrometry. Annals of Clinical and Laboratory Science 46(3): 242-246, 2016

Quantification of carbamazepine and carbamazepine epoxide in serum by high-performance liquid-chromatography. Journal of Pharmacy & Pharmacology 52(Supplement): 141, September, 2000

Quantification of carbamazepine and carbamazepine 10 11 epoxide by high performance liquid chromatography for therapeutic control. Revista de Farmacia e Bioquimica da Universidade de Sao Paulo 25(2): 95-108, 1989

Determination of carbamazepine and its metabolite carbamazepine-10,11-epoxide in serum with gas-chromatography mass spectrometry. Methods and Findings in Experimental and Clinical Pharmacology 23(9): 481-485, 2002

Determination of lamotrigine, carbamazepine and carbamazepine epoxide in human serum by gas chromatography mass spectrometry. European Journal of Clinical Chemistry and Clinical Biochemistry 35(10): 755-759, 1997

Simultaneous determination of zonisamide, carbamazepine and carbamazepine-10,11-epoxide in infant serum by high-performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis 41(4): 1386-1390, 2006

Simultaneous quantitation of salivary carbamazepine, carbamazepine-10,11-epoxide, phenytoin and phenobarbitone by high-performance liquid chromatography. Journal of Chromatography 496(1): 147-154, 1989

Quantification of carbamazepine, carbamazepine-10,11-epoxide, phenytoin and phenobarbital in plasma samples by stir bar-sorptive extraction and liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis 48(2): 428-434, 2008

Simultaneous determination of phenytoin, carbamazepine, and 10,11-carbamazepine epoxide in human plasma by high-performance liquid chromatography with ultraviolet detection. Journal of Pharmaceutical & Biomedical Analysis 16(7): 1233-1240, March, 1998

Development, validation and clinical application of an online-SPE-LC-HRMS/MS for simultaneous quantification of phenobarbital, phenytoin, carbamazepine, and its active metabolite carbamazepine 10,11-epoxide. Talanta 158: 77-88, 2016

A reverse phase high performance liquid chromatography method for simultaneous estimation of melatonin, carbamazepine epoxide and carbamazepine simultaneously in serum. Indian Journal of Physiology and Pharmacology 50(4): 427-430, 2007

Simultaneous determination of carbamazepine and carbamazepine 10,11-epoxide by using microcolumn HPLC: study of pharmacokinetics of carbamazepine in a volunteer. Biomedical Chromatography 11(1): 36-41, 1997