+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Assay of hepatitis B virus DNA by polymerase chain reaction and its relationship to pre-S- and S-encoded viral surface antigens



Assay of hepatitis B virus DNA by polymerase chain reaction and its relationship to pre-S- and S-encoded viral surface antigens



Hepatology 13(1): 158-166



The polymerase chain reaction was evaluated as a diagnostic tool in 72 chronic hepatitis B virus carriers. Hepatitis B virus DNA was detectable in the serum of HBsAg-positive virus carriers using aliquots as small as 100 al. The detection limit for cloned hepatitis B virus DNA was 100 ag. Primer pairs for different regions of the HBV genome resulted in different sensitivity. Detection of the amplified hepatitis B virus DNA by Southern blotting and subsequent scintillation counting or densitometry allowed a semiquantitative assay. Using several primer pairs in parallel for optimal detection, all HBeAg-positive HBsAg carriers, 80% of HBe antibody-positive symptomatic HBsAg carriers and 57% of asymptomatic HBe antibody-positive HBsAg carriers were found to have hepatitis B virus DNA in the serum. During antiviral therapy hepatitis B virus DNA disappeared by the polymerase chain reaction assay in patients who became HBeAg negative, but polymerase chain reaction detected a relapse earlier than did the conventional dot blot. Pre-S antigens were assayed in serum and liver samples from most chronic carriers by enzyme-linked immunosorbent assay and/or immunoblot. Although most viremic carriers were strongly positive for pre-S1 and pre-S2 antigens, some hepatitis B virus DNA-positive HBsAg carriers did not have detectable pre-S antigens, and vice versa. Our data show that assay of hepatitis B virus DNA in the serum by polymerase chain reaction is by far more proficient than by dot blot and that it cannot be replaced by serological assays of HBeAg or pre-S antigen.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039330167

Download citation: RISBibTeXText

PMID: 1988337

DOI: 10.1002/hep.1840130123


Related references

Polymerase chain reaction analysis for viral nucleic acids in acute sporadic hepatitis patients negative for serum hepatitis B surface antigen and antibodies to hepatitis C virus. Zhonghua Yi Xue Za Zhi 58(6): 379-384, 1996

Lessons from a multicentre study of the detectability of viral genomes based on a two-round quality control of GB virus C (GBV-C)/hepatitis G virus (HGV) polymerase chain reaction assay. Journal of Virological Methods 85(1-2): 117-124, 2000

Detection of hepatitis B viral DNA by polymerase chain reaction in patients with hepatitis B surface antigen. Journal of Medical Virology 30(3): 159-162, 1990

Antibodies to hepatitis C virus and hepatitis C virus RNA in Chinese blood donors determined by ELISA, recombinant immunoblot assay and polymerase chain reaction. Chinese Medical Journal 106(3): 171-174, 1993

Hepatitis C virus antibodies and hepatitis C virus RNA in Chinese blood donors determined by ELISA, recombinant immunoblot assay and polymerase chain reaction. Apmis. 100(9): 851-855, 1992

Prevalence of hepatitis B or C virus infection in patients with fulminant viral hepatitis: An analysis using polymerase chain reaction. Journal of Hepatology 24(3): 258-264, 1996

Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. Proceedings of the National Academy of Sciences of the United States of America 86(1): 312-316, 1989

Significance of hepatitis B viremia levels determined by a quantitative polymerase chain reaction assay in patients with hepatitis B e antigen-negative chronic hepatitis B virus infection. American Journal of Gastroenterology 98(10): 2261-2267, 2003

Detection of hepatitis C virus by polymerase chain reaction and response to interferon-alpha therapy: relationship to genotypes of hepatitis C virus. Hepatology 16(2): 293-299, 1992

Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection. Virology Journal 8: 227, 2011

Simultaneous extraction of hepatitis C virus (HCV), hepatitis B virus, and HIV-1 from plasma and detection of HCV RNA by a reverse transcriptase-polymerase chain reaction assay designed for screening pooled units of donated blood. Transfusion 39(10): 1111-1119, 1999

Clinical evaluation of a single reaction, diagnostic polymerase chain reaction assay for the detection of hepatitis C virus RNA. Journal Of Hepatology. 24(1): 33-37, 1996

Polymerase chain reaction assay for detection of hepatitis B virus. American Journal of Clinical Pathology 95(4): 442-444, 1991

In chronic hepatitis b loss of serum hepatitis b viral dna hbv dna detectable by polymerase chain reaction pcr usually occurs at the time of loss of hepatitis b surface antigen hbsag. Hepatology 12(4 PART 2): 846, 1990

In-house developed reverse transcription-polymerase chain reaction assay vs modified Roche Amplicor RT-PCR assay for hepatitis C virus RNA detection. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 26, 1996