Section 40
Chapter 39,337

Assignment of protein NMR spectra in the light of homonuclear 3D spectroscopy: an automatable procedure based on 3D TOCSY-TOCSY and 3D TOCSY-NOESY

Oschkinat, H.; Holak, T.A.; Cieslar, C.

Biopolymers 31(6): 699-712


ISSN/ISBN: 0006-3525
PMID: 1932568
DOI: 10.1002/bip.360310615
Accession: 039336152

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Automated assignment of proteins is greatly simplified using data from 3D-nmr spectra. A strategy is presented which makes use of 3D-TOCSY-TOCSY and 3D-TOCSY-NOESY; its potential is demonstrated with the example of the spectra of BPTI. The discussion of the potential of 3D-nmr includes the introduction of a simple graph for the description of the information content of multidimensional nmr spectra.

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