+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Attenuation of late simian virus 40 mRNA synthesis is enhanced by the agnoprotein and is temporally regulated in isolated nuclear systems

Attenuation of late simian virus 40 mRNA synthesis is enhanced by the agnoprotein and is temporally regulated in isolated nuclear systems

Molecular and Cellular Biology 5(6): 1327-1334

Studies were performed to verify the physiological significance of attenuation in the life cycle of simian virus 40 and the role of agnoprotein in this process. For these purposes, nuclei were isolated at various times after infection and incubated in vitro in the presence of [alpha-32P]UTP under the standard conditions which lead to attenuation. Attenuation was evident by the production of a 94-nucleotide attenuator RNA, revealed by gel electrophoresis. In parallel, the synthesis of agnoprotein was studied at various times after infection by labeling the cells for 3 h with [14C]arginine, lysing them, and analyzing the labeled proteins by gel electrophoresis. Both attenuation and the synthesis of agnoprotein were predominant towards the end of the infectious cycle. At earlier times, there was almost no attenuation and no synthesis of agnoprotein. Moreover, there was almost no attenuation even at the latest times after infection in nuclei isolated from cells infected with simian virus 40 deletion mutants that do not synthesize agnoprotein. Finally, analysis by dot blot hybridization showed higher amounts of cytoplasmic viral RNA in cells infected with an agnoprotein gene insertion mutant, delta 79, that does not produce agnoprotein, compared with cells infected with wild-type virus. The present studies indicate that attenuation is temporally regulated and suggest that agnoprotein enhances attenuation in isolated nuclei and that may also enhance it in vivo.

(PDF emailed within 0-6 h: $19.90)

Accession: 039346050

Download citation: RISBibTeXText

PMID: 2993860

DOI: 10.1128/mcb.5.6.1327

Related references

Attenuation of late sv 40 messenger rna synthesis is enhanced by the agnoprotein and is temporally regulated in isolated nuclear systems. Molecular & Cellular Biology 5(6): 1327-1334, 1985

Simian virus 40 agnoprotein facilitates perinuclear-nuclear localization of VP1, the major capsid protein. Journal of Virology 60(3): 1055-1061, 1986

Translational control of synthesis of simian virus 40 late proteins from polycistronic 19S late mRNA. Journal of Virology 62(9): 3182-3192, 1988

Evidence for simian virus 40 late transcriptional control: mixed infections of wild-type simian virus 40 and a late leader deletion mutant exhibit trans effects on late viral RNA synthesis. Journal of Virology 42(3): 798-803, 1982

Control of late simian virus 40 transcription by the attenuation mechanism and transcriptionally active ternary complexes are associated with the nuclear matrix. Journal of Molecular Biology 172(4): 467-487, 1984

Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus. Journal of Virology 60(3): 1098-1106, 1986

Control of simian virus 40 gene expression at the levels of RNA synthesis and processing: thermally induced changes in the ratio of the simian virus 40 early mRNA's and proteins. Journal of Virology 35(1): 157-164, 1980

Expression of novel proteins by polyomaviruses and recent advances in the structural and functional features of agnoprotein of JC virus, BK virus, and simian virus 40. Journal of Cellular Physiology 2018, 2018

Subcellular localization of the simian virus 40 agnoprotein. Journal of Virology 45(1): 428-433, 1983

Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence. Journal of Virology 50(1): 163-173, 1984

Role of the agnoprotein in regulation of simian virus 40 replication and maturation pathways. Journal of Virology 61(3): 937-939, 1987

Simian virus 40 late mRNA leader sequences involved in augmenting mRNA accumulation via multiple mechanisms, including increased polyadenylation efficiency. Journal of Virology 65(12): 6677-6685, 1991

Attenuation of Simian Varicella Virus Infection by Enhanced Green Fluorescent Protein in Rhesus Macaques. Journal of Virology 92(7), 2018

A 21-base pair DNA fragment directs transcription attenuation within the simian virus 40 late leader. Journal of Biological Chemistry 266(20): 13019-13027, 1991

Leakage of nuclear transcripts late in simian virus 40-infected CV1 cells: quantitation of spliced and unspliced late 19S RNAs. Journal of Virology 36(2): 595-600, 1980