+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Axonal transport of two major components of the ubiquitin system: free ubiquitin and ubiquitin carboxyl-terminal hydrolase PGP 9.5

Axonal transport of two major components of the ubiquitin system: free ubiquitin and ubiquitin carboxyl-terminal hydrolase PGP 9.5

Brain Research 548(1-2): 292-299

Ubiquitin (Ub), a stress protein thought to target abnormal proteins for degradation, is present in abnormal structures that occur in neuronal perikarya and axons of degenerative diseases including Alzheimer disease. To begin to assess the role of the Ub system in the axon, we studied expression and axonal transport of Ub and other stress proteins, as well as of Ub carboxyl-terminal hydrolase PGP 9.5, in the rat visual system in normal conditions and following heat-shock (HS). In the retina, both the constitutive and inducible forms of HSPs 70 were expressed under normal conditions, while in the superior colliculus the inducible form was detected only following HS. Ub, PGP 9.5 and HSPs 70 were transported in the axon exclusively with the slow component b (SCb), known to carry cytoskeletal and cytoplasmic proteins. The exceedingly long time needed for stress proteins to reach distant axonal locales at the rate of SCb (approximately 3 mm/day) makes it unlikely that they could contribute significantly to the stress response at those sites.

(PDF emailed within 0-6 h: $19.90)

Accession: 039359794

Download citation: RISBibTeXText

PMID: 1714333

DOI: 10.1016/0006-8993(91)91135-n

Related references

Synthesis and characterization of ubiquitin ethyl ester, a new substrate for ubiquitin carboxyl-terminal hydrolase. Biochemistry 25(21): 6644-6649, 1986

Mechanism of ubiquitin carboxyl-terminal hydrolase. Borohydride and hydroxylamine inactivate in the presence of ubiquitin. Journal of Biological Chemistry 261(22): 10210-7, 1986

Ubiquitin carboxyl-terminal hydrolase acts on ubiquitin carboxyl-terminal amides. Journal of Biological Chemistry 260(13): 7903-7910, 1985

Ubiquitin C-Terminal Hydrolase L1 is required for regulated protein degradation through the ubiquitin proteasome system in kidney. Kidney International 93(1): 110-127, 2017

Role of the Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence. Orl; Journal for Oto-Rhino-Laryngology and Its Related Specialties 79(3): 153-163, 2017

Non enzymatic synthesis of ubiquitin protein conjugates and ubiquitin carboxyl terminal derivatives. Federation Proceedings 46(6): 2086, 1987

Ubiquitin carboxyl-terminal peptides. Substrates for ubiquitin activating enzyme. Journal of Biological Chemistry 263(11): 5016-5019, 1988

Ubiquitin carboxy-terminal hydrolase L1 mediates ubiquitin stability and function in neurons. Neuroscience Research Suppl. (26): S8, 2002( ), 2003

Mutant ubiquitin (UBB+1) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3). Febs Letters 585(16): 2568-2574, 2011

Characterizing substrate selectivity of ubiquitin C-terminal hydrolase-L3 using engineered α-linked ubiquitin substrates. Biochemistry 53(51): 8031-8042, 2015

Characterization of the Ubiquitin C-Terminal Hydrolase and Ubiquitin-Specific Protease Families in Rice ( Oryza sativa ). Frontiers in Plant Science 9: 1636, 2018

Ubiquitin terminal hydrolase identity with pgp95 and immunolocalization in ubiquitin containing inclusion bodies in brain but not liver. Neuropathology & Applied Neurobiology 16(3): 260, 1990

Capillary electrophoresis assay for ubiquitin carboxyl-terminal hydrolases with chemically synthesized ubiquitin-valine as substrate. Analytical Biochemistry 247(2): 305-309, 1997

Expression of ubiquitin-related enzymes in the suprachiasmatic nucleus with special reference to ubiquitin carboxy-terminal hydrolase UchL1. Biomedical Research 26(2): 43-49, 2005

Ubiquitin C-terminal hydrolase as a regulator of ubiquitin level. Society for Neuroscience Abstracts 27(1): 517, 2001