+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Beta-adrenergic stimulation of pineal N-acetyltransferase: adenosine 3':5'-cyclic monophosphate stimulates both RNA and protein synthesis

Beta-adrenergic stimulation of pineal N-acetyltransferase: adenosine 3':5'-cyclic monophosphate stimulates both RNA and protein synthesis

Proceedings of the National Academy of Sciences of the United States of America 72(6): 2107-2111

The lag period in the induction of rat pineal N-acetyltransferase (arylamine acetyltransferase or acetyl-CoA:arylamine N-acetyltransferase EC by catecholamines via the beta-adrenergic receptor varies with the length of exposure of the rat to light or darkness. If rats have been exposed to light and reduced sympathetic nerve activity for more than 12 hr, this lag period is 1-2 hr long. Under these conditions, actinomycin D completely blocks the induction of N-acetyltransferase by isoproterenol and by dibutyryl adenosine 3':5'-cyclic monophosphate (cyclic AMP). In contrast, if enzyme activity is caused to fall by brief exposure to light at night when N-acetyltransferase activity is high, reinduction by catecholamines occurs almost immediately. In this case, actinomycin D does not block the reinduction of N-acetyltransferase by isoproterenol or by dibutyryl cyclic AMP. Cycloheximide blocks N-acetyltransferase induction under all conditions tested. Thus, new protein synthesis is always required for N-acetyltransferase induction; however, the requirement for RNA synthesis is variable, and contributes to the length of the lag period for induction. It is postulated that both beta-adrenergic stimulation and dibutyryl cyclic AMP act intracellularly at two separate sites in the induction of pineal N-acetyltransferase. One site is in the stimulation of transcription, and the other is in the stimulation of post-transcriptional events.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 039380855

Download citation: RISBibTeXText

PMID: 166379

DOI: 10.2307/64657

Related references

Role of the beta adrenergic receptor in the elevation of adenosine cyclic 3',5'-monophosphate and induction of serotonin N-acetyltransferase in rat pineal glands. Molecular Pharmacology 9(2): 184-190, 1973

Blockade by ouabain or elevated potassium ion concentration of the adrenergic and adenosine cyclic 3',5'-monophosphate-induced stimulation of pineal serotonin N-acetyltransferase activity. Molecular Pharmacology 11(3): 241-255, 1975

Adrenergic-adenosine 3',5'-monophosphate regulation of serotonin N-acetyltransferase activity and the temporal relationship of serotonin N-acetyltransferase activity synthesis of 3H-N-acetylserotonin and 3H-melatonin in the cultured rat pineal gland. Journal of Pharmacology and Experimental Therapeutics 186(3): 516-527, 1973

Rat pineal adenosine cyclic 3',5'-monophosphate phosphodiesterase activity: modulation in vivo by a beta adrenergic receptor. Molecular Pharmacology 11(5): 552-557, 1975

Cyclic 3',5'-adenosine monophosphate stimulation of chloramphenicol-acetyltransferase synthesis in bacterial cellular systems. Biulleten' Eksperimental'noi Biologii i Meditsiny 80(10): 65-66, 1975

Endothelin-1 stimulates contraction of rat glomerular mesangial cells and potentiates beta-adrenergic-mediated cyclic adenosine monophosphate accumulation. Journal of Clinical Investigation 85(3): 790-797, 1990

Adenosine effects on the rat pineal gland in vitro: cyclic adenosine monophosphate levels, N-acetyltransferase, and thyroxine type II 5'-deiodinase activities and melatonin production. Journal of Pineal Research 11(1): 1-6, 1991

Triiodothyronine increases contractility independent of beta-adrenergic receptors or stimulation of cyclic-3',5'-adenosine monophosphate. Anesthesiology 82(4): 1004-1012, 1995

Modulation of myogenic afferent arteriolar vasoconstriction by beta adrenergic stimulation and cyclic adenosine monophosphate c amp. Journal of the American Society of Nephrology 2(3): 523, 1991

Two responsive elements in the angiotensin converting enzyme promoter may confer beta adrenergic and cyclic adenosine monophosphate stimulation. Circulation 94(8 Suppl. ): I106, 1996

Prostaglandin E2 increases adenosine 3',5'-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro. Molecular and Cellular Endocrinology 23(2): 151-159, 1981

Fatty acid degradation in Escherichia coli: requirement of cyclic adenosine monophosphate and cyclic adenosine monophosphate receptor protein for enzyme synthesis. Journal of Bacteriology 117(3): 1178-1183, 1974

Alpah-adrenergic receptor modulation of beta-adrenergic, adenosine and prostaglandin E1 increased adenosine 3':5'-cyclic monophosphate levels in primary cultures of glia. Journal of Cyclic Nucleotide Research 4(1): 15-26, 1978

Adenosine stimulates adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate accumulation in rat pinealocytes: evidence for a role for adenosine in pineal neurotransmission. Endocrinology 125(4): 2150-2157, 1989

Effects on fluorine analogs of norepinephrine on stimulation of cyclic adenosine 3',5'-monophosphate and binding to beta-adrenergic receptors in intact pinealocytes. Biochemical Pharmacology 30(10): 1085-1089, 1981