Biosynthesis and secretion of bovine growth hormone in Escherichia coli under the control of the secretory vector containing a promoter and signal region of the alkaline phosphatase gene

Tsfasman, I.M.; Nesmeianova, V.G.; Gorbulev, V.G.; Rubtsov, P.M.; Skriabin, K.G.

Molekuliarnaia Biologiia 23(2): 422-430


ISSN/ISBN: 0026-8984
PMID: 2671671
Accession: 039401855

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A recombinant plasmid carrying a bovine growth hormone gene fused with the regulatory and signal regions of the alkaline phosphatase gene of E. coli was constructed. The bovine growth hormone gene expression as well as protein partial processing and secretion into the periplasm have been shown to take place under phosphate starvation, i.e. conditions of alkaline phosphatase derepression.