+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Calcium-dependent salivary agglutinin with reactivity to various oral bacterial species



Calcium-dependent salivary agglutinin with reactivity to various oral bacterial species



Infection and Immunity 53(1): 173-178



Human parotid agglutinins from three individuals were isolated by adsorption to and desorption from strains of Streptococcus mutans belonging to serotypes a, b, c, d, and e and strains of Lactobacillus casei, Actinomyces viscosus, and Streptococcus sanguis. The desorption was achieved by suspending centrifuged saliva-coated microorganisms in 10 mM phosphate buffer (pH 6.8) containing 0.154 M sodium chloride. After another centrifugation, agglutinin activity was recovered in the supernatants. The L. casei strain was not agglutinated by any of the agglutinin extracts or by saliva, but all the other strains were agglutinated to a variable extent. However, all strains, including the nonagglutinating L. casei strain, adsorbed and desorbed agglutinins active for other strains. The agglutinin extracts from S. mutans serotype c, S. sanguis, and A. viscosus were purified and characterized by electrophoretic and immunological techniques. The purified preparations were positively stained for protein and carbohydrate, and the molecular weights were estimated to be 440,000. All agglutinin extracts needed calcium in the range of 0.1 to 0.5 mM to be active, and for a single strain, all agglutinins gave the same degree of agglutination, indicating that the isolated agglutinins may be of the same molecular species, a hypothesis that was also confirmed by the preliminary characterization of the purified agglutinins. This type of agglutinin, which seems to exert its activity among various bacterial species, could be important in mediating bacterial coaggregation and thus may add to the effect of specific agglutinins in the clearance of bacteria from the human mouth.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039442827

Download citation: RISBibTeXText

PMID: 3087878


Related references

Differentiation and interaction of secretory immunoglobulin A and a calcium-dependent parotid agglutinin for several bacterial strains. Infection and Immunity 55(2): 288-292, 1987

Salivary agglutinin and secretory IgA reactions with oral streptococci. Scandinavian Journal of Dental Research 86(6): 430-443, 1978

Complement activation by salivary agglutinin is secretor status dependent. Biological Chemistry 396(1): 35-43, 2015

Calcium-binding properties of SSP-5, the Streptococcus gordonii M5 receptor for salivary agglutinin. Infection and Immunity 62(12): 5220-5226, 1994

Effect of calcium on reactions between a salivary agglutinin and a serotype c strain of Streptococcus mutans. Journal of Oral Pathology 10(4): 269-275, 1981

Evaluation of salivary calcium and salivary parathyroid levels in postmenopausal women with and without oral dryness. Contemporary Clinical Dentistry 4(4): 488-492, 2014

The Human Glycoprotein Salivary Agglutinin Inhibits the Interaction of DC-SIGN and Langerin with Oral Micro-Organisms. Journal of Innate Immunity 8(4): 350-361, 2017

Reactivity of red cells from patients with diabetes mellitus with a dextrose dependent pan agglutinin. Transfusion (Bethesda) 20(5): 629, 1980

Age dependent reactivity of peripheral lymphocytes to phyto hem agglutinin p evaluated by their acid phosphatase activity. Res Journal Of The Reticuloendothelial Societyppl: 11a, 1975

Streptococcus mutans SMU.623c codes for a functional, metal-dependent polysaccharide deacetylase that modulates interactions with salivary agglutinin. Journal of Bacteriology 191(1): 394-402, 2009

Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces. Infection and Immunity 61(8): 3199-3208, 1993

Salivary bacterial fingerprints of established oral disease revealed by the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) technique. Journal of Oral Microbiology 8: 30170, 2016

Quercetin-dependent scavenging of reactive nitrogen species derived from nitric oxide and nitrite in the human oral cavity: interaction of quercetin with salivary redox components. Archives of Oral Biology 51(8): 629-639, 2006

Salivary agglutinin/DMBT1SAG expression is up-regulated in the presence of salivary gland tumors. Journal of Dental Research 83(7): 567-571, 2004

Hydrogen ion dependent calcium uptake into an inositol 1 4 5 trisphosphate sensitive calcium pool of acinar cells from rat salivary glands. Heilmann, C. Falk Symposium, 48. Calcium-Dependent Processes In The Liver; Symposium, Titisee, West Germany, June 13-15, . Xv 310p. Kluwer Academic Publishers: Dordrecht, Netherlands; Boston, Massachusetts, Usa. Illus. 121-136, 1988