EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline Ti plasmid pTiC58



Characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline Ti plasmid pTiC58



Journal of Bacteriology 170(4): 1759-1767



Overlapping segments of pTiC58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine A and B Agrobacterium tumefaciens strain C58. All of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines A and B. Transposon Tn3-HoHo1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines A and B and agrocin 84. Such insertions were found to cluster within a 4.4-kilobase region. Analysis of beta-galactosidase activity in these insertion mutants suggested a single transcriptional unit regulated at the transcriptional level by agrocinopines A and B. The smallest DNA fragment subcloned from the region to confer all three activities was 8.5 kilobases long. This subclone was still properly regulated, indicating that the regulatory gene is closely linked to the locus. The data are consistent with a single operon encoding catabolism of agrocinopines A and B and conferring sensitivity to agrocin 84. Based on these results, we support the locus name acc, for agrocinopine catabolism.

(PDF emailed within 1 workday: $29.90)

Accession: 039517936

Download citation: RISBibTeXText

PMID: 2832379



Related references

Characterization of the acc operon from the nopaline-type Ti plasmid pTiC58, which encodes utilization of agrocinopines A and B and susceptibility to agrocin 84. Journal of Bacteriology 179(23): 7559-7572, 1997

Characterization of the acc operon from the nopaline-type Ti plasmid pTiC58, which encodes utilization of agrocinopines A and B susceptibility to agrocin 84. Journal of Bacteriology 179(23): 7559-7572, 1997

The agrocinopine catabolic region of agrobacterium tumefaciens ti plasmid ptic58 encodes at least five genes required for opine and agrocin 84 transport. Phytopathology 82(9): 992, 1992

Acc the agrocinopine catabolic region of agrobacterium tumefaciens ti plasmid ptic58 encodes multiple genes required for opine and agrocin 84 transport. Abstracts of the General Meeting of the American Society for Microbiology 92: 203, 1992

Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport. Journal of Bacteriology 175(17): 5575-5584, 1993

Characterization of a nopaline-induced gene for a 40 kDa protein in the nopaline catabolic (noc) region of Ti plasmid pTiC58. Biochimica et Biophysica Acta, Gene Structure and Expression 1174(3): 303-304, 1993

Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping. Plasmid 3(2): 193-211, 1980

Characterization of the trb region on the nopaline-type Ti plasmid pTiC58 of Agrobacterium tumefaciens. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 496, 1996

Characterization of the virA virulence gene of the nopaline plasmid, pTiC58, of Agrobacterium tumefaciens. Molecular Microbiology 3(9): 1237-1246, 1989

Characterization of the tra3 region on the nopaline-type Ti plasmid pTiC58 of Agrobacterium tumefaciens. Abstracts of the General Meeting of the American Society for Microbiology 94(0): 245, 1994

Modification of T-DNA of nopaline Ti plasmid by intermediary vector and utilization of agrocin 84 sensitivity as simple criterion of conjugation transfer of modified Ti plasmid. Folia Microbiologica 31(2): 86-93, 1986

The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid 3(2): 212-230, 1980

The functional organization of the nopaline Agrobacterium tumefasciens plasmid pTiC58. Plasmid 3(2): 212-230, 1980

Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58. Journal of Bacteriology 169(4): 1529-1536, 1987