+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Characterization of JHMV variants isolated from rat brain and cultured neural cells after wild type JHMV infection

Characterization of JHMV variants isolated from rat brain and cultured neural cells after wild type JHMV infection

Advances in Experimental Medicine and Biology 218: 343-349

ISSN/ISBN: 0065-2598

PMID: 2829546

After intracerebral inoculation of wild type (wt) JHMV into 4 to 5 week-old Lewis rats, only variants with larger mRNA3 were selectively propagated and no wt JHMV was reisolated from the brain. Detailed analysis of a cloned virus from infected rat brain, c1-2, showed that the virus had larger mRNAs 2, 2a and 3 as compared with those of wt JHMV, while there was no such difference for other mRNAs. The E2 glycoprotein of a variant virus was also shown to be larger as compared with that of wt JHMV. Such selective replication of variants were also observed in neural cell culture after infection with wt JHMV. However, these variants isolated from the brain of infected rat (c1-2) and from infected neural cells (CNS virus) differed from each other in the amounts of mRNAs 2 and 2a as well as 65 K protein. All of these data suggest that the viruses with larger E2 glycoprotein have the growth advantage in rat brain cells, which could be responsible for acute encephalitis of rats after infection with wt JHMV.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 039518701

Download citation: RISBibTeXText

Related references

Characterization of a variant virus isolated from neural cell culture after infection of mouse coronavirus JHMV. Virology 155(1): 267-270, 1986

Intramuscular injection of plasmid DNA expressing mRNA7 coding the nucleocapsid protein of JHMV partially protected mice against acute infection with JHMV. Advances in Experimental Medicine and Biology 440: 693-699, 1998

Induction and recruitment of myelin-specific CD4 T cells during persistent JHMV infection. Journal of Neuroimmunology 275(1-2): 80-81, 2014

Neurovirulence of six different murine coronavirus JHMV variants for rats. Virus Research 20(1): 45-58, 1991

Receptor-independent spread of a highly neurotropic murine coronavirus JHMV strain from initially infected microglial cells in mixed neural cultures. Journal of Virology 79(10): 6102-6110, 2005

Neurovirulence for rats of the JHMV variants escaped from neutralization with the S1-specific monoclonal antibodies. Advances in Experimental Medicine and Biology 380: 185-187, 1995

The role of CD4+ T cells in entry of CD8+ CTL into the central nervous system during acute infection with the neurotropic JHMV strain of mouse hepatitis virus. Journal of Cellular Biochemistry Suppl 1995(19A): 307, 1995

Transcription and translation of proinflammatory cytokines following JHMV infection. Advances in Experimental Medicine and Biology 380: 173-178, 1995

Receptor-independent spread of a neurotropic murine coronavirus MHV-JHMV in mixed neural culture. Advances in Experimental Medicine and Biology 581: 327-330, 2006

Apoptosis of JHMV-specific CTL in the CNS in the absence of CD4+ T cells. Advances in Experimental Medicine and Biology 440: 425-430, 1998

Viral evolution and CTL epitope stability during JHMV infection in the central nervous system. Advances in Experimental Medicine and Biology 440: 751-757, 1998

Epigenetic factors influencing the morphogenesis of primary neural cell cultures and the concomitant effects on establishing JHMV infections. Advances in Experimental Medicine and Biology 276: 655-667, 1990

Pathogenic murine coronaviruses. III. Biological and biochemical characterization of temperature-sensitive mutants of JHMV. Virology 94(2): 385-399, 1979

An immunological analysis of natural resistance to mouse hepatitis virus (JHMV strain) infection in C3H mice. Brazilian Journal of Medical and Biological Research 20(1): 115-123, 1987

In vivo and in vitro models of demyelinating disease. Possible relationship between induction of regulatory subunit from cAMP dependent protein kinases and inhibition of JHMV replication in cultured oligodendrocytes. Advances in Experimental Medicine and Biology 276: 261-266, 1990