+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of blood mononuclear cells producing IFN alpha following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)



Characterization of blood mononuclear cells producing IFN alpha following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)



Research in Immunology 141(2): 141-151



Porcine blood mononuclear cells (PBMC) were shown to produce interferon-alpha (IFN alpha) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFN alpha producer cells. The production of IFN alpha was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039521256

Download citation: RISBibTeXText

PMID: 2167506


Related references

Proteome profile of swine testicular cells infected with porcine transmissible gastroenteritis coronavirus. Plos one 9(10): E110647, 2014

Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins. Veterinary Research 25(1): 29-36, 1994

Interferon-alpha-producing cells are localized in gut-associated lymphoid tissues in transmissible gastroenteritis virus (TGEV) infected piglets. Veterinary Research 32(1): 71-79, 2001

Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus. American journal of veterinary research 54(2): 254-259, 1993

Cellular immune responses of pigs after primary inoculation with porcine respiratory coronavirus or transmissible gastroenteritis virus and challenge with transmissible gastroenteritis virus. Veterinary Immunology & Immunopathology 48(1-2): 35-54, 1995

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus. Journal of General Virology 74: 1795-1804, 1993

Induction of protective immunity against transmissible gastroenteritis virus after exposure of neonatal pigs to porcine respiratory coronavirus. American Journal of Veterinary Research 57(2): 157-162, 1996

Isotype-specific antibody-secreting cells to transmissible gastroenteritis virus and porcine respiratory coronavirus in gut- and bronchus-associated lymphoid tissues of suckling pigs. Journal of Immunology 150(9): 3990-4000, 1993

Antigenic characterization of porcine respiratory coronavirus with the aid of monoclonal antibodies against transmissible gastroenteritis virus. Journees de la recherche porcine en France: 0th) 89-93, 1988

Characterization of the effector cells in antibody dependent and spontaneous cell mediated cytotoxicity in swine against target cells infected with transmissible gastroenteritis virus. Research in Veterinary Science 41(1): 70-75, 1986

Transmissible gastroenteritis virus and porcine respiratory coronavirus: molecular characterization of the S gene using cDNA probes and nucleotide sequence analysis. Advances in Experimental Medicine and Biology 342: 43-48, 1993

Single amino acid changes in viral glycoprotein m affect induction of alpha interferon by the coronavirus transmissible gastroenteritis virus. Journal of Virology 66(2): 743-749, 1992

Molecular characterization and pathogenesis of transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCV) field isolates co-circulating in a swine herd. Archives of Virology 145(6): 1133-1147, 2000

Characterization of the IgA and subclass IgG responses to neutralizing epitopes after infection of pregnant sows with the transmissible gastroenteritis virus or the antigenically related porcine respiratory coronavirus. Journal of General Virology 75: 2585-2593, 1994

Sequence analysis of the leader rna of two porcine coronaviruses transmissible gastroenteritis virus and porcine respiratory coronavirus. Virus Genes 4(4): 289-302, 1990