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Characterization of monoclonal antibodies against human leukocyte 5-lipoxygenase



Characterization of monoclonal antibodies against human leukocyte 5-lipoxygenase



Prostaglandins Leukotrienes and Essential Fatty Acids 45(4): 259-265



Four mouse monoclonal IgG1 antibody-producing cell lines (5LO-1, 5LO-2, 5LO-3, 5LO-4), produced against highly purified human leukocyte 5-lipoxygenase have been characterized. The monoclonal antibodies produced by these cell lines exhibited differential reactivity against 5-lipoxygenase as determined by ELISA and immunoprecipitation analyses. Monoclonal antibodies 5LO-2 and 5LO-3 inhibited the activity of recombinant human leukocyte 5-lipoxygenase in a dose-dependent manner. This inhibition was selective for 5-lipoxygenase activity since these monoclonal antibodies did not inhibit human leukocyte 15-lipoxygenase or porcine leukocyte 12-lipoxygenase.

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Accession: 039523335

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PMID: 1603817

DOI: 10.1016/0952-3278(92)90081-s


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